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Proteomics of Outermembrane Vesicles (OMV) from Acinetobacter baumannii DS002

ABSTRACT: Outer membrane vesicles from A. baumannii DS002 were isolated using Diafilteration and Ultracentrifugation. The proteins of OMVs were separated on a 12.5% SDS-PAGE gel. The protein bands were excised and in-gel digetion was carried out using trypsin. The resultant peptides were analysed on LC-ESI-MS/MS. The proteins were identified with the help of Proteome Discoverer software.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Acinetobacter Baumannii

SUBMITTER: Medicharla V. Jagannadham

LAB HEAD: Dayananda Siddavattam

PROVIDER: PXD026751 | Pride | 2022-03-15

REPOSITORIES: Pride

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			Action	DRS
	DS002-1-5-ESC-QE-1AbOMV-60min-090321-MVJ-Ganeshwari-UOH-EXTERNAL-LAYERS.xlsx	Xlsx
	DS002-ESC-QE-1-1-1p5-AbOMV-60min-090321-MVJ-Ganeshwari-UOH-EXTERNAL-LAYERS.xlsx	Xlsx
	ESC-QE-1-1-AbOMV-60min-090321-MVJ-Ganeshwari-UOH-EXTERNAL.raw	Raw
	ESC-QE-1-2-AbOMV-60min-090321-MVJ-Ganeshwari-UOH-EXTERNAL.raw	Raw
	ESC-QE-1-3-AbOMV-60min-090321-MVJ-Ganeshwari-UOH-EXTERNAL.raw	Raw

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Publications

Outer Membrane Vesicles of <i>Acinetobacter baumannii</i> DS002 Are Selectively Enriched with TonB-Dependent Transporters and Play a Key Role in Iron Acquisition.

Dhurve Ganeshwari G Madikonda Ashok Kumar AK Jagannadham Medicharla Venkata MV Siddavattam Dayananda D

Microbiology spectrum 20220310 2

Outer membrane vesicles (OMVs) of Acinetobacter baumannii DS002 carry proteins which perform selective biological functions. The proteins involved in cell wall/membrane biogenesis and inorganic ion transport and metabolism occupied a significant portion of the 302 proteins associated with OMVs. Interestingly, the TonB-dependent transporters (TonRs), linked to the active transport of nutrients across the energy-deprived outer membrane, are predominant among proteins involved in inorganic ion tran ...[more]

PMID: 35266817

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