Dataset Information

Cell-matrix adhesion contributes to permeability control in human colon organoids

ABSTRACT: Background and aims: Cell-cell adhesion structures (desmosomes and, especially, tight junctions) are known to play important roles in control of transepithelial permeability in the colon. The involvement of cell-matrix interactions in permeability control is less clear. The goals of the present study were to: i) determine if disruption of colon epithelial cell interactions with the extracellular matrix alters permeability control and ii) determine if increasing the elaboration of protein components of cell-matrix adhesion complexes improves permeability control and mitigates the effects of cell-matrix disruption. Results: Treatment of colon organoids with Aquamin® increased the expression of multiple basement membrane and hemidesmosomal proteins as well as keratin 8 and 18. TEER values were higher in the presence of Aquamin® than they were under control conditions. Conclusions: These findings provide evidence that cell-matrix interactions contribute to permeability control in the colon. They suggest that the elaboration of proteins important to cell-matrix interactions can be increased in human colon organoids by exposure to a multi-mineral natural product. Increasing the elaboration of such proteins may help to mitigate the consequences of disrupting cell-matrix interactions on permeability control.

INSTRUMENT(S): Orbitrap Fusion

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Colon

DISEASE(S): Disease Free

SUBMITTER: Muhammad Aslam

LAB HEAD: Muhammad N Aslam

PROVIDER: PXD026923 | Pride | 2022-03-14

REPOSITORIES: Pride

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Dataset's files

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			Action	DRS
	PRF_F_2019_J_VARA_12_UCR126_1.raw	Raw
	PRF_F_2019_J_VARA_12_UCR126_1_8.msf	Msf
	PRF_F_2019_J_VARA_12_UCR126_2.raw	Raw
	PRF_F_2019_J_VARA_12_UCR126_3.raw	Raw
	PRF_F_2019_J_VARA_12_UCR126_4.raw	Raw

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Publications

Cell-Matrix Interactions Contribute to Barrier Function in Human Colon Organoids.

Varani James J McClintock Shannon D SD Aslam Muhammad N MN

Frontiers in medicine 20220310

The importance of cell-matrix adhesion to barrier control in the colon is unclear. The goals of the present study were to: (i) determine if disruption of colon epithelial cell interactions with the extracellular matrix alters permeability control measurement and (ii) determine if increasing the elaboration of protein components of cell-matrix adhesion complexes can mitigate the effects of cell-matrix disruption. Human colon organoids were interrogated for transepithelial electrical resistance (T ...[more]

PMID: 35360746

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