Proteomics

Dataset Information

0

The human proteome project, C-HPP 18 chromosome project


ABSTRACT: The Russian part of C-HPP is aimed to identify proteins encoded by the 18 human chromosome. In this project, 3 liver tissue samples were studied by standart Shotgun and 2 dimensional liquid chromatography approach to enhance the sensitivity of the MS method. The total amount of proteins found in samples is 5022 and the proteins encoded by the 18 human chromosome is 64.

INSTRUMENT(S): Q Exactive HF-X

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Liver

SUBMITTER: Nikita Vavilov  

LAB HEAD: Victor G. Zgoda

PROVIDER: PXD026997 | Pride | 2022-05-20

REPOSITORIES: Pride

Dataset's files

Source:
Action DRS
120421NV_Liver01.raw Raw
120421NV_Liver01_210412192635.raw Raw
120421NV_Liver01_210412212834.raw Raw
130521X18Liver2.raw Raw
130521X18Liver2F01.raw Raw
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Publications

Deep proteomic dataset of human liver samples obtained by two-dimensional sample fractionation coupled with tandem mass spectrometry.

Vavilov Nikita E NE   Ilgisonis Ekaterina V EV   Lisitsa Andrey V AV   Ponomarenko Elena A EA   Farafonova Tatiana E TE   Tikhonova Olga V OV   Zgoda Victor G VG   Archakov Alexander I AI  

Data in brief 20220312


The data was acquired from 3 normal human liver tissues by LC-MS methods. The tissue liver samples from male subjects post mortem were obtained from ILSBio LLC (https://bioivt.com/). Liver tissue was frozen in liquid nitrogen, transported and shipped on dry ice. The proteins were extracted and purified followed up by trypsin hydrolysis. The peptide mixture was aliquoted and analyzed by different LC-MS approaches: one-dimensional shotgun LC-MS, two-dimensional LC-MS, two-dimensional SRM SIS (Sele  ...[more]

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