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The human proteome project, C-HPP 18 chromosome project

ABSTRACT: The Russian part of C-HPP is aimed to identify proteins encoded by the 18 human chromosome. In this project, 3 liver tissue samples were studied by standart SRM and 2 dimensional liquid chromatography approach to enhance the sensitivity of the MS method. The total amount of proteins found in samples encoded by the 18 human chromosome is 118. UPS1 used as calibration and to measure the sensitivity of SRM method.

INSTRUMENT(S): 6495A Triple Quadrupole LC/MS

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Liver

SUBMITTER: Nikita Vavilov

LAB HEAD: Victor G. Zgoda

PROVIDER: PXD028510 | Pride | 2022-05-19

REPOSITORIES: Pride

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Dataset's files

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			Action	DRS
	Liver_1_2D_SRM.zip	Other
	Liver_1_SRM.zip	Other
	Liver_3_2D_SRM.zip	Other
	Liver_3_SRM.zip	Other
	Liver_5_2D_SRM.zip	Other

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Publications

Deep proteomic dataset of human liver samples obtained by two-dimensional sample fractionation coupled with tandem mass spectrometry.

Vavilov Nikita E NE Ilgisonis Ekaterina V EV Lisitsa Andrey V AV Ponomarenko Elena A EA Farafonova Tatiana E TE Tikhonova Olga V OV Zgoda Victor G VG Archakov Alexander I AI

Data in brief 20220312

The data was acquired from 3 normal human liver tissues by LC-MS methods. The tissue liver samples from male subjects post mortem were obtained from ILSBio LLC (https://bioivt.com/). Liver tissue was frozen in liquid nitrogen, transported and shipped on dry ice. The proteins were extracted and purified followed up by trypsin hydrolysis. The peptide mixture was aliquoted and analyzed by different LC-MS approaches: one-dimensional shotgun LC-MS, two-dimensional LC-MS, two-dimensional SRM SIS (Sele ...[more]

PMID: 35345844

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