Proteomics

Dataset Information

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Proteomic profiling of glioblastoma stem cells, human astrocyte, and T98G glioblastoma cell line

ABSTRACT: With HiRIEF LC-MS/MS shotgun proteomics, we analysed 6 patient-derived glioblastoma stem cells (BT stem cells) and compared them to an astrocyte line (CliniSciences, Guidonia Montecelio, Italy) and a more differentiated glioblastoma cell line (T98G). Each of the 8 cell line sample was run in triplicates in a total of three TMT10 sets, assigning each replicate in a separate TMT10 set, using 2 internal standards per set. The three TMT10 sets were ran in two experiments, first on immobilized pH gradient (IPG) 3-10 isoelectric focusing (IEF) strips, and then on IPG 3.7-4.9 IEF strips.

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Malignant Cell, Astrocyte Cell Line, Glioblastoma Multiforme Cancer Stem Cell, Astrocyte, Glioblastoma Cell Line

DISEASE(S): Glioblastoma,Glioblastoma Classical Subtype,Brain Cancer,Idh-wildtype Glioblastoma

SUBMITTER: Haris Babačić  

LAB HEAD: Janne Lehtiö

PROVIDER: PXD027335 | Pride | 2022-12-23

REPOSITORIES: Pride

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Dataset's files

Source:
Action DRS
GBM_BT_stem_cells_TMT10plex_250ug_HiRIEF_IPG_3.7-4.9_genes_table_.txt Txt
GBM_BT_stem_cells_TMT10plex_250ug_HiRIEF_IPG_3.7-4.9_labelling_scheme.txt Txt
GBM_BT_stem_cells_TMT10plex_250ug_HiRIEF_IPG_3.7-4.9_peptides_table.txt Txt
GBM_BT_stem_cells_TMT10plex_250ug_HiRIEF_IPG_3.7-4.9_proteins_table.txt Txt
GBM_BT_stem_cells_TMT10plex_250ug_HiRIEF_IPG_3.7-4.9_target_psmtable.txt Txt
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Publications

Glioblastoma (GBM) cancer stem cells (GSCs) contribute to GBM's origin, recurrence, and resistance to treatment. However, the understanding of how mRNA expression patterns of GBM subtypes are reflected at global proteome level in GSCs is limited. To characterize protein expression in GSCs, we performed in-depth proteogenomic analysis of patient-derived GSCs by RNA-sequencing and mass-spectrometry. We quantified > 10 000 proteins in two independent GSC panels and propose a GSC-associated proteomi  ...[more]

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