Proteomics

Dataset Information

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Combined Metabolic and Chemical (CoMetChem) labeling using stable isotopes

ABSTRACT: Histone acetylation is an important, reversible posttranslational protein modification and hallmark of epigenetic regulation. However, little is known about the dynamics of reversible hisotne acetylation, due to the lack of analytical methods that can capture site-specific acetylation and deacetylation reactions. We present a new approach that combines metabolic and chemical labeling (CoMetChem) using uniformly 13C-labeled glucose and stable isotope labeled acetic anhydride, which allows to quantify site-specific lysine acetylation dynamics in tryptic peptides using high-resolution mass spectrometry.

INSTRUMENT(S): Orbitrap Fusion Lumos, Q Exactive Plus

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Cell Culture, Macrophage

DISEASE(S): Disease Free

SUBMITTER: Anna-Sophia Egger  

LAB HEAD: Marcel Kwiatkowski

PROVIDER: PXD027338 | Pride | 2021-11-02

REPOSITORIES: Pride

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Dataset's files

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Action DRS
T05_C_replicate_1.raw Raw
T05_C_replicate_2.raw Raw
T05_C_replicate_3.raw Raw
T05_MS-275_replicate_1.raw Raw
T05_MS-275_replicate_2.raw Raw
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Publications

Combined Metabolic and Chemical (CoMetChem) Labeling Using Stable Isotopes-a Strategy to Reveal Site-Specific Histone Acetylation and Deacetylation Rates by LC-MS.

van Pijkeren Alienke A   Dietze Jörn J   Brotons Alejandro Sánchez AS   Egger Anna-Sophia AS   Lijster Tim T   Barcaru Andrei A   Hotze Madlen M   Kobler Philipp P   Dekker Frank J FJ   Horvatovich Peter P   Melgert Barbro N BN   Ziegler Mathias M   Thedieck Kathrin K   Heiland Ines I   Bischoff Rainer R   Kwiatkowski Marcel M  

Analytical chemistry 20210914 38

Histone acetylation is an important, reversible post-translational protein modification and a hallmark of epigenetic regulation. However, little is known about the dynamics of this process, due to the lack of analytical methods that can capture site-specific acetylation and deacetylation reactions. We present a new approach that combines metabolic and chemical labeling (CoMetChem) using uniformly 13C-labeled glucose and stable isotope-labeled acetic anhydride. Thereby, chemically equivalent, ful  ...[more]

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