Dataset Information

Comparative analysis of ChAdOx1 nCoV-19 and Ad26.COV2.S SARS-CoV-2 vector vaccines

ABSTRACT: Vector-based SARS-CoV-2 vaccines such as AstraZeneca's ChAdOx1 nCoV-19 have been associated with vaccine-induced thrombosis (TTS/VITT). We studied ChAdOx1 nCov-19 (AstraZeneca) and Ad26.COV2.S (Johnson & Johnson) and compared the vaccines using proteomics with mass spectrometric measurements. We could demonstrate that ChAdOx1 nCoV-19 contained significant amounts of human proteins in addition to adenoviral proteins. This was not the case for Ad26.COV2.S. Among the detected human proteins, proteasome subunits beta 1/delta and beta 5/MB1 were identified. In summary, both studied SARS-CoV-2 vaccines are markedly different concerning their content of contaminating proteins, which is most likely a determining factor for different frequencies of TTS/VITT after their application.

INSTRUMENT(S): Orbitrap Exploris 480

ORGANISM(S): Homo Sapiens (human)

DISEASE(S): Covid-19

SUBMITTER: Christian Hentschker

LAB HEAD: Uwe Völker

PROVIDER: PXD027344 | Pride | 2023-05-04

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	20210412_human_uniprot_reviewed_20394_seq_210125_with_cAdY25_NC_017825_E4region_repl_with_hAD5_000008_plus_SPIKE_SARS-cov2.fasta	Fasta
	20210412_human_uniprot_reviewed_20394_seq_210125_with_hAd26_plus_plus_hAd5_E4orf6_7__SPIKE_SARS-cov2.fasta	Fasta
	20210517_Exp_CH_HEK-P41_15-625ng.raw	Raw
	20210517_Exp_CH_HEK-P41_250ng.raw	Raw
	20210517_Exp_CH_HEK-P41_31-25ng.raw	Raw

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Publications

Comparative analysis of ChAdOx1 nCoV-19 and Ad26.COV2.S SARS-CoV-2 vector vaccines.

Michalik Stephan S Siegerist Florian F Palankar Raghavendra R Franzke Kati K Schindler Maximilian M Reder Alexander A Seifert Ulrike U Cammann Clemens C Wesche Jan J Steil Leif L Hentschker Christian C Gesell-Salazar Manuela M Reisinger Emil E Beer Martin M Endlich Nicole N Greinacher Andreas A Völker Uwe U

Haematologica 20220401 4

Vector-based SARS-CoV-2 vaccines have been associated with vaccine- induced thrombosis with thrombocytopenia syndrome (VITT/TTS), but the causative factors are still unresolved. We comprehensively analyzed the ChAdOx1 nCoV-19 (AstraZeneca) and Ad26.COV2.S (Johnson and Johnson) vaccines. ChAdOx1 nCoV-19 contains significant amounts of host cell protein impurities, including functionally active proteasomes, and adenoviral proteins. A much smaller amount of impurities was found in Ad26.COV2.S. Plat ...[more]

PMID: 35045692

Dataset Information

Comparative analysis of ChAdOx1 nCoV-19 and Ad26.COV2.S SARS-CoV-2 vector vaccines

Dataset's files

Publications

Comparative analysis of ChAdOx1 nCoV-19 and Ad26.COV2.S SARS-CoV-2 vector vaccines.

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