Dataset Information

Quantification of isoaspartate levels in human serum albumin of blood samples

ABSTRACT: We measured the isoaspartate levels in human serum albumin of blood samples from 20 patients with Alzheimer's disease and 20 healthy controls.

INSTRUMENT(S): Orbitrap Fusion

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Blood Plasma

SUBMITTER: Jijing Wang

LAB HEAD: Roman A. Zubarev

PROVIDER: PXD027563 | Pride | 2022-02-17

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	ADScanner3_01_1.dat	Other
	ADScanner3_01_1.raw	Raw
	ADScanner3_01_1_PeptMatrix.txt	Txt
	ADScanner3_01_1_PeptMatrix.xlsx	Xlsx
	ADScanner3_01_1_ProtMatrix.txt	Txt

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Publications

First Immunoassay for Measuring Isoaspartate in Human Serum Albumin.

Wang Jijing J Lundström Susanna L SL Seelow Sven S Rodin Sergey S Meng Zhaowei Z Astorga-Wells Juan J Jia Qinyu Q Zubarev Roman A RA

Molecules (Basel, Switzerland) 20211105 21

Isoaspartate (isoAsp) is a damaging amino acid residue formed in proteins mostly as a result of spontaneous deamidation of asparaginyl residues. An association has been found between isoAsp in human serum albumin (HSA) and Alzheimer's disease (AD). Here we report on a novel monoclonal antibody (mAb) 1A3 with excellent specificity to isoAsp in the functionally important domain of HSA. Based on 1A3 mAb, an indirect enzyme-linked immunosorbent assay (ELISA) was developed, and the isoAsp occupancy i ...[more]

PMID: 34771115

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