Ontology highlight
ABSTRACT:
INSTRUMENT(S): Orbitrap Fusion
ORGANISM(S): Homo Sapiens (human)
TISSUE(S): Blood Plasma
SUBMITTER: Jijing Wang
LAB HEAD: Roman A. Zubarev
PROVIDER: PXD027563 | Pride | 2022-02-17
REPOSITORIES: Pride
Wang Jijing J Lundström Susanna L SL Seelow Sven S Rodin Sergey S Meng Zhaowei Z Astorga-Wells Juan J Jia Qinyu Q Zubarev Roman A RA
Molecules (Basel, Switzerland) 20211105 21
Isoaspartate (isoAsp) is a damaging amino acid residue formed in proteins mostly as a result of spontaneous deamidation of asparaginyl residues. An association has been found between isoAsp in human serum albumin (HSA) and Alzheimer's disease (AD). Here we report on a novel monoclonal antibody (mAb) 1A3 with excellent specificity to isoAsp in the functionally important domain of HSA. Based on 1A3 mAb, an indirect enzyme-linked immunosorbent assay (ELISA) was developed, and the isoAsp occupancy i ...[more]