Proteomics

Dataset Information

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Analysis of RNA polymerase II interacting proteins in wild-type and spt6-1004 yeast cells.


ABSTRACT: The histone chaperone Spt6 is involved in promoting elongation of RNA polymerase II (RNAPII), maintaining chromatin structure, regulating co-transcriptional histone modifications, and controlling mRNA processing. These diverse functions of Spt6 are partly mediated through its interactions with RNAPII and other factors in the transcription elongation complex. In this study, we used mass spectrometry to characterize the differences in RNAPII interacting factors between wild-type cells and those depleted for Spt6, leading to the identification of proteins that depend on Spt6 for their interaction with RNAPII. In all, eight samples were processed - four genotypes (1. SPT6, RPB3-untagged; 2. SPT6, RPB3-tagged; 3. spt6-1004, RPB3-untagged; 4. spt6-1004; RPB3-tagged) in biological duplicates.

INSTRUMENT(S): Orbitrap Fusion

ORGANISM(S): Saccharomyces Cerevisiae (baker's Yeast)

SUBMITTER: Rajaraman Gopalakrishnan  

LAB HEAD: Fred Winston

PROVIDER: PXD027708 | Pride | 2021-12-05

REPOSITORIES: Pride

Dataset's files

Source:
Action DRS
3188_z04397_tc-o238g-setB_MS3_static-TMT.mzid.gz Mzid
checksum.txt Txt
z04397_tc-o238g-setB_MS3.mgf Mgf
z04397_tc-o238g-setB_MS3.raw Raw
z04397_tc-o238g-setB_MS3_static-TMT.mzXML Mzxml
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Publications

The histone chaperone Spt6 is required for normal recruitment of the capping enzyme Abd1 to transcribed regions.

Gopalakrishnan Rajaraman R   Winston Fred F  

The Journal of biological chemistry 20210917 4


The histone chaperone Spt6 is involved in promoting elongation of RNA polymerase II (RNAPII), maintaining chromatin structure, regulating cotranscriptional histone modifications, and controlling mRNA processing. These diverse functions of Spt6 are partly mediated through its interactions with RNAPII and other factors in the transcription elongation complex. In this study, we used mass spectrometry to characterize the differences in RNAPII-interacting factors between wildtype cells and those depl  ...[more]

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