Proteomics

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TipNrich, a tip-based N-terminal proteome enrichment method

ABSTRACT: Protein N-termini provide valuable information such as half-life of protein, but one of hurdles to study N-terminome is that it requires large amount of sample, but this requirement might be problematic for analyzing the limited samples such as low number of cells of clinical biopsies. In this regard, we developed tip-based N-terminome enrichment method, tipNrich. Our method, the scale-down version of our previously introduced N-terminome enrichment method iNrich, provides simple and fast N-terminone analysis platform even for analyzing small amount of protein. Our method could be utilized either for quality check of protein products or as a diagnostic tool for human diseases related to N-terminal proteoforms. We applied our method to various recombinant single proteins, protein mixture, and plasma samples of wild-type transthyretin amyloidosis (WTTA) patients.

INSTRUMENT(S): LTQ Orbitrap, LTQ, Q Exactive

ORGANISM(S): Bos Taurus (bovine) Escherichia Coli Bacillus Subtilis Mus Musculus (mouse)

TISSUE(S): Blood Plasma

DISEASE(S): Wild-type Amyloidosis

SUBMITTER: Seonjeong Lee  

LAB HEAD: Cheolju Lee

PROVIDER: PXD027961 | Pride | 2022-12-05

REPOSITORIES: Pride

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200201_Senile_gluC_C01.raw Raw
200201_Senile_gluC_C02.raw Raw
200201_Senile_gluC_C03.raw Raw
200201_Senile_gluC_C04.raw Raw
200201_Senile_gluC_C05.raw Raw
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Publications

tipNrich: A Tip-Based N-Terminal Proteome Enrichment Method.

Lee Seonjeong S   Ju Shinyeong S   Kim Seok Jin SJ   Choi Jin-Oh JO   Kim Kihyun K   Kim Darae D   Jeon Eun-Seok ES   Lee Cheolju C  

Analytical chemistry 20211006 42

The mass spectrometry-based analysis of protein post-translational modifications requires large amounts of sample, complicating the analysis of samples with limited amounts of proteins such as clinical biopsies. Here, we present a tip-based N-terminal analysis method, tipNrich. The entire procedure is processed in a single pipette tip to minimize sample loss, which is so highly optimized to analyze small amounts of proteins, even femtomole-scale of a single protein. With tipNrich, we investigate  ...[more]

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