Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Functional analysis of Arabidopsis LSH8 protein involved in ABA signaling pathway

ABSTRACT: Functional analysis of Arabidopsis LSH8 protein involved in ABA signaling pathway

INSTRUMENT(S): Orbitrap Fusion Lumos, 4700 Proteomics Analyzer

ORGANISM(S): Arabidopsis Thaliana (mouse-ear Cress)

TISSUE(S): Whole Body

SUBMITTER: 李志芳

LAB HEAD: Zhifang Li

PROVIDER: PXD027962 | Pride | 2022-02-17

REPOSITORIES: Pride

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	lzf-A65-fra.msf	Msf
	lzf-A65-fra1.raw	Raw
	lzf-A65-fra10.raw	Raw
	lzf-A65-fra2.raw	Raw
	lzf-A65-fra3.raw	Raw

Items per page:

1 - 5 of 22

Publications

Arabidopsis <i>LSH8</i> Positively Regulates ABA Signaling by Changing the Expression Pattern of ABA-Responsive Proteins.

Zou Jinpeng J Li Zhifang Z Tang Haohao H Zhang Li L Li Jingdu J Li Yuhong Y Yao Nan N Li Yaxing Y Yang Deguang D Zuo Zecheng Z

International journal of molecular sciences 20210925 19

Phytohormone ABA regulates the expression of numerous genes to significantly affect seed dormancy, seed germination and early seedling responses to biotic and abiotic stresses. However, the function of many ABA-responsive genes remains largely unknown. In order to improve the ABA-related signaling network, we conducted a large-scale ABA phenotype screening. LSH, an important transcription factor family, extensively participates in seedling development and floral organogenesis in plants, but whet ...[more]

PMID: 34638657

Similar Datasets

A phosphoproteomic analysis of ABA induced protein phosphorylation revealed that Serine 71 is a major phosphosite for bZIP72 in SAPK10-mediated ABA signaling

Project description:we focused on the profiling of rice young seedling phosphosites, phosphopeptides and phosphorylation intensity dynamics in response to ABA by employing a label-free, MS-based phosphoproteomic approach

2018-10-27 | PXD004705 | Pride

Reciprocal regulation of the TOR kinase and ABA receptor balances plant growth and stress response

Project description:We found that TOR kinase regulates the plant environmental stress responses through phosphorylation at a conserved serine residue of Abscisic acid receptor PYR1/PYLs/RCARs. The phosphorylation inhibits the function of PYLs and prevents the binding of PYLs to abscisic acid and its downstream substrates of PP2C, which blocks the ABA and stress signaling. Our results suggest that plant utilizes this mechanism as a hub to regulate the ABA-mediate stress responses and growth recovery

2017-12-01 | PXD003746 | Pride

Transcriptional profiling by array of Arabidopsis ABA auxotroph to identify early downstream targets of transcription in response to a small dose of abscisic acid (ABA)

Project description:Abscisic acid (ABA) is a plant hormone that is important in responding to various environmental stresses. Using an ABA auxotroph in Arabidopsis (aba2-2) as the plant material, we would like to identify early downstream targets of transcription in response to a small dose of ABA, 1 uM. We also added cycloheximide to preferentially obtain immediate targets of ABA addition. We believe that using a sensitized background of an ABA auxotroph would yield a set of genes that are very closely regulated by ABA at the transcriptional level. This data set will be used for network analysis of ABA signaling.

2011-10-30 | E-MEXP-3404 | biostudies-arrayexpress

Arabidopsis hy2 mutant LC-MS/MS

Project description:Salt stress is a critical factor of abiotic stress in agricultural production that significantly affects seed germination and early seedling development. In this study, we characterized the function of the Arabidopsis Long Hypocotyl 2(HY2) gene in NaCl signaling. The hy2 mutant was NaCl-insensitive, whereas HY2-overexpressing lines shows NaCl-hypersensitive phenotypes during seed germination. Induced by exogenous NaCl, the transcription and the protein level of HY2 were up regulated, and HY2 positively mediated the expression of downstream stress-related genes of RD29A, RD29B and DREB2A. With further quantitative proteomics, we got the patterns of 7,391 proteins under salt stress, and identified 215 differentially regulated proteins (DRPs) specifically regulated by HY2. According to GO enrichment analysis, these proteins are mainly involved in ion homeostasis, hormone response, reactive oxygen species(ROS) metabolic, photosynthesis and detoxification pathway to response salt stress . These results direct the pathway of HY2 participating salt stress, and provide new insights for the plant to resist salt stress.

2022-02-17 | PXD027204 | Pride

Abscisic acid (ABA) application to Populus × canescens in hydroponics

Project description:4 weeks old rooted plantlets of P. × canescens (Clone INRA717 1-B4) were cultivated in hydroponics in 2 l pots in Long Ashton nutrient solution in a culture room for 8 weeks before treatments started. Three treatments were applied to the plants: control treatment (-ABA), continuous 100 µM ABA treatment (+ABA) and discontinuous 100 µM ABA treatment (±ABA). ABA was fed to +ABA plants during the whole treatment period of 30 days. ABA was fed to ±ABA plants for three days in two weeks. Developing xylem and mature xylem were collected separately during the harvest and shortly frozen in liquid nitrogen. RNA was extracted from these samples and followed by RNA-sequencing.

2019-01-20 | E-MTAB-7587 | biostudies-arrayexpress

RNA-seq analysis of date-palms treated with stress hormone ABA compared to controls

Project description:Date palms are extremely stress tolerant. The aim of the experiment was to evaluate, how date palm leaves react to application of the drought stress hormone ABA on the transcriptional level, and to compare the resulting data with those obtained from the stress sensitive Arabidopsis thaliana. The experiment thus should help us to understand how date palms manage their stress tolerance in comparison to sensitive plants. Single cut pinneates from date palm leaves were treated for 2 h with CO2-free air in darkness, then illuminated for 4 h at a photon flux rate of 620 mol m2 s-1. Following this pre-incubation, 25 M ABA was fed via the petiole. After 2 h, RNA was extracted from the pinneates. Controls were treated equally with ABA-free solution.

2017-07-31 | E-MTAB-5261 | biostudies-arrayexpress

Expression data of T87 Arabidopsis cultured cells in the presence of ABA, SA, JA, ABA+SA, or ABA+JA for 3hr or 24hr

Project description:Plant hormones involved in environmental stresses, namely abscisic acid (ABA), salicylic acid (SA), and jasmonic acid (JA), have been shown to interact with each other in a complex manner. To address the network of the hormone interactions, we have investigated the changes in expression under multiple hormone treatments, ABA+SA and ABA+JA. We chose cultured cells to remove the difference in the response to hormones among developmental cells or tissues. The cells were treated for 3hr and 24hr to see the rapid or transient response and steady-state response. The obtained data indicate that ABA and SA affect antagonistically, but these hormones affected many genes collaboratively. Indeed, according to the microarray data, there are many genes that responded only to ABA+SA. In addition, the ABA+SA responsive genes also responded to ABA+JA. These data suggest that hormone crosstalk is more complicated than expected and that more systematic analysis is required to untangle the hormone crosstalk network. To investigate the hormonal interactions, Arabidopsis T87 cultured cells were exposed to ABA, SA, or JA alone, or two hormones simultaneously, ABA+SA or ABA+JA, for 3hr and 24 hr. Comparing the data among those treatments, the relationships among these hormones were deduced.

2011-12-01 | E-GEOD-28600 | biostudies-arrayexpress

mRNA-seq of ros1-3 and ros1-4 with ABA treatment

Project description:To survey transcriptome changes by the mutations of a DNA demethylase ROS1 responding to a phytohormone abscisic acid, we performed the Next-gen sequencing (NGS) associated RNA-seq analysis. Two ROS1 knockout lines (ros1-3, ros1-4; Penterman et al. 2007 [PMID: 17409185]) with the wild-type Col line (wt) were subjected. Three samples (ros1-3, ros1-4 and wt), biological triplicates, ABA or mock treatment, using Illumina HiSeq 2500 system

2016-01-15 | E-GEOD-65016 | biostudies-arrayexpress

Expression comparasion of rice seedlings of Osabl1 mutant and WT under ABA treatment

Project description:Expression profiles of seedling roots of rice mutant Osabl1 and wild-type under ABA treatment Mutant and wild-type expression profiling were compared and each has two biological repeats.

2011-10-12 | E-GEOD-18529 | biostudies-arrayexpress

Transcriptional profiling of DEX-inducible SNRK3.15 seedlings in the presence of ABA

Project description:Transcriptional profiling of a DEX-inducible SNRK3.15 seedlings in the presence of ABA.

2012-03-01 | E-MEXP-3397 | biostudies-arrayexpress