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Downstream Molecules of YKL-40 in Regulating Apoptosis in BMDM

ABSTRACT: An iTRAQ-based proteomics analysis was performed on BMDM to screen out the potential downstream target molecules of YKL-40 in regulating macrophage apoptosis.

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Cell Culture, Macrophage

DISEASE(S): Cardiovascular System Disease

SUBMITTER: Wei Huan

LAB HEAD: Lefeng Qu

PROVIDER: PXD028305 | Pride | 2022-02-15

REPOSITORIES: Pride

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Publications

YKL-40 Aggravates Early-Stage Atherosclerosis by Inhibiting Macrophage Apoptosis in an Aven-dependent Way.

Huan Wei W Yandong Liu L Chao Wang W Sili Zou Z Jun Bai B Mingfang Liao L Yu Chen C Lefeng Qu Q

Frontiers in cell and developmental biology 20211207

<b>Objective:</b> programmed cell removal in atherosclerotic plaques plays a crucial role in retarding lesion progression. Macrophage apoptosis has a critical role in PrCR, especially in early-stage lesions. YKL-40 has been shown to be elevated as lesions develop and is closely related to macrophages. This study aimed to determine the effect of YKL-40 on regulating macrophage apoptosis and early-stage atherosclerosis progression. <b>Research design and Methods:</b> The correlations among the exp ...[more]

PMID: 34950656

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2022-02-17 | PXD027323 | Pride

Expression data from infected with Neisseria gonorrhoeae (GC) and uninfected bone marrow derived macrophages from wild type (C67BL/6) and Nod2 knock out mice

Project description:In this dataset we include the data obtained from 3 hour stimulation with Neisseria gonorrhoeae (GC) of bone marrow macrophages(BMDM) from wild type (C57BL/6) and Nod2 knock out mice (in C57BL/6 background). Murine BMDM from C57BL/6 mice or NOD2 deficient mice on C57BL/6 background, plated in 6 well plates, were infected with GC at MOI 10:1 or left uninfected. At 3 hrs after infection, cells lysates were made and RNA was isolated. All conditions were done in triplicates(3 wells per condition. The experiment was done once). Whole genome microarray analysis was carried out using the Affymetrix GeneChip Gene 1.0 ST Array System. A 2-way ANOVA was applied to analyze the effects of genotype and infection by GC as well as the interaction between genotype and infection by GC. Raw affinity data was normalized using a Robust Multichip Analysis (RMA) method at probe level Microarray Core, Boston University Medical Center

2012-09-01 | E-GEOD-37179 | biostudies-arrayexpress

Expression data from pig BMDM treated with salmonella LPS

Project description:Mouse bone marrow-derived macrophages (BMDM) grown in macrophage colony-stimulating factor (CSF-1) have been used widely in studies of macrophage biology and the response to toll-like receptor agonists. We investigated whether similar cells could be derived from the domestic pig. Cultivation of pig bone marrow cells for 5-7 days in presence of rhCSF-1 generated a pure population of BMDM that expressed the usual macrophage markers (CD14, CD16, CD163, CD172a), are potent phagocytic cells and produced tumor necrosis factor (TNF) in response to lipopolysaccharide (LPS). Bone marrow cells could be stored frozen and thawed, providing a renewable resource. We profiled gene expression in pig BMDM from outbred animals (Large-White Landrace F1cross) responding to LPS using Affymetrix microarrays. The temporal cascade of inducible and repressible genes more closely-resembled human than mouse macrophages, and included genes involved in tryptophan metabolism (IDO, KYN), lymphoattractant chemokines (CCL20) and the vitamin D3-converting enzyme Cyp27B1. Conversely, pig BMDM, like human macrophages, did not induce genes involved in arginine metabolism, nor did they produce nitric oxide. The data establish pig BMDM as an alternative tractable model for the study of macrophage transcriptional control. RNA for gene expression analysis was collected at time points 0, 2, 7 and 24 hours post LPS stimulation (100ng/ml). Each time point included BMDM from the same three pigs and each cell culture was replicated. The replicate of the pig3_24h was not suitable for RNA analysis. Therefore, a total of 23 microarrays were hybridized.

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Host cell subversion by Toxoplasma GRA16, a HAUSP- and PP2A-interacting protein exported to the host cell nucleus

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