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Drosophila nicotinic acetylcholine receptor subunits and their native interactions with insecticidal peptide toxins

ABSTRACT: Description Drosophila melanogaster nicotinic acetylcholine receptors (nAChRs) are ligand-gated ion channels that present a target for insecticides. However, a better understanding of in vioreceptor subunit composition is needed for effective design of insecticides. Alpha-neurotxins are known as peptide toxins capable to block nAChRs by binding to its target subunits. To facilitate the analysis of nAChRs we used a CRISPR/Cas9 strategy to generate null alleles for all ten nAChR subunit genes. We studied interactions of nAChRs to a snake toxin by using styrene maleic acid lipid particles (SMALPs) extraction method. For the null alleles which are impaired in locomotor activities we examined the binding of nAChRs incorporated into SMALPs to alpha bungarotoxin by employing mass spectrometry. We show native ligand interactions to Dalpha5, Dalpha6 and Dalpha7 subunits and reveal glycosylation sites of Dalpha5 and Dalpha7 by a glycoproteomic approach. Finally, we report the localization of fluorescent tagged Dalpha6 across nervous system development

INSTRUMENT(S): Q Exactive

ORGANISM(S): Drosophila Melanogaster (fruit Fly)

TISSUE(S): Brain

SUBMITTER: Kathryn Lilley

LAB HEAD: Kathryn S. Lilley

PROVIDER: PXD028484 | Pride | 2021-10-11

REPOSITORIES: Pride

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Source:

			Action	DRS
	1_Gel_flow_through.mzML	Mzml
	1_Gel_flow_through.mzid.gz	Mzid
	1_Gel_flow_through.raw	Raw
	1_minus_BTX_minus_GLY.mzML	Mzml
	1_minus_BTX_minus_GLY.mzid.gz	Mzid

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Drosophila nicotinic acetylcholine receptor subunits and their native interactions with insecticidal peptide toxins

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