Dataset Information

Sarcoglycan interactions in murine muscle membranes

ABSTRACT: The sarcoglycan complex (SC) is part of a network that links the striated muscle cytoskeleton to the basal lamina across the sarcolemma. The SC coordinates changes in phosphorylation and Ca++-flux during mechanical deformation, and these processes are disrupted with loss-of-function mutations in gamma-sarcoglycan (Sgcg) that cause Limb Girdle Muscular Dystrophy 2C/R5. To gain insight into how the SC mediates mechano-signaling in muscle, we utilized LC-MS/MS proteomics to identify SC-associated proteins in immunoprecipitates from enriched sarcolemmal fractions. Criteria for inclusion were selective co-immunoprecipitation with anti-Sgcg from C57BL/6 control muscle, but under-representation in parallel experiments with Sgcg-null muscle or with non-specific IgG. Co-immunoprecipitation from archvillin-deleted muscle (Svil-null) was also evaluated.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Plasma Membrane, Muscle Fibre, Gastrocnemius

DISEASE(S): Autosomal Recessive Limb-girdle Muscular Dystrophy Type 2c

SUBMITTER: Scott Shaffer

LAB HEAD: Elizabeth J. Luna

PROVIDER: PXD028584 | Pride | 2022-02-16

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	20180829_Luna_GSGxWT-_1_.msf	Msf
	20180829_Luna_GSGxWT-_1_.mzid.gz	Mzid
	20180829_Luna_GSGxWT-_1_.mzid.gz_20180829_Luna_GSGxWT-_1_.MGF	Mzid
	20180829_Luna_GSGxWT.raw	Raw
	20180829_Luna_IgGxWT_control-_1_.msf	Msf

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Publications

Novel γ-sarcoglycan interactors in murine muscle membranes.

Smith Tara C TC Vasilakos Georgios G Shaffer Scott A SA Puglise Jason M JM Chou Chih-Hsuan CH Barton Elisabeth R ER Luna Elizabeth J EJ

Skeletal muscle 20220122 1

<h4>Background</h4>The sarcoglycan complex (SC) is part of a network that links the striated muscle cytoskeleton to the basal lamina across the sarcolemma. The SC coordinates changes in phosphorylation and Ca<sup>++</sup>-flux during mechanical deformation, and these processes are disrupted with loss-of-function mutations in gamma-sarcoglycan (Sgcg) that cause Limb girdle muscular dystrophy 2C/R5.<h4>Methods</h4>To gain insight into how the SC mediates mechano-signaling in muscle, we utilized LC ...[more]

PMID: 35065666

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