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Radiotherapy changes the salivary proteome in head and neck cancer patients: evaluation before, during and after treatment

ABSTRACT: Objectives: Salivary glands are affected during radiotherapy in the head and neck region, leading to a reduction in salivary flow and changes its composition. Besides negatively affecting the oral soft tissues, this can also lead to dental impairment. Thus, we evaluated the effect of radiotherapy in the proteomic profile of the saliva in patients with head-and-neck-cancer (HNC). Materials and methods: HNC patients had their saliva collected before (BRT), during (2-5 weeks; DRT) and after (3-4 months; ART) radiotherapy. Saliva was also collected from healthy volunteers (control; C). Samples were processed for proteomic analysis. Results: In total 1.055 proteins were identified, among which 46 were common to all groups, while 86, 86, 286 and 395 were exclusively found in C, BRT, DRT and ART, respectively. Remarkably, alpha-enolase was increased 35-fold DRT compared with BRT, while proline-rich proteins were decreased. ART there was a 16-fold increase in scaffold attachment factor-B1 and a 3-fold decrease in alpha-enolase and several cystatins. When compared with C, salivary proteins of BRT patients showed increases cystatin-C, lysozyme C, histatin-1 and proline-rich proteins. Conclusion/Clinical revelance: Both HNC and radiotherapy remarkably change the salivary protein composition. Altogether, our results, for the first time, suggest investigating alpha-enolase levels in saliva DRT in future studies as a possible biomarker and strategy to predict the efficiency of the treatment. Moreover, our data provide important insights for designing dental products that are more effective for these patients and contribute to a better understanding of the progressive changes in salivary proteins induced by radiotherapy.

INSTRUMENT(S): Xevo G2 Q-Tof, nanoACQUITY UPLC

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Saliva

DISEASE(S): Head And Neck Cancer

SUBMITTER: Talita Ventura

LAB HEAD: Talita Mendes Oliveira Ventura

PROVIDER: PXD028594 | Pride | 2021-10-12

REPOSITORIES: Pride

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			Action	DRS
	20190905_TALITA_R1.zip	Other
	20190905_TALITA_R2.zip	Other
	20190905_TALITA_R3.zip	Other
	R1_IA_unstimulated_saliva.zip	Other
	R2_IA_unstimulated_saliva.zip	Other

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Radiotherapy changes the salivary proteome in head and neck cancer patients: evaluation before, during and after treatment

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