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Omics score: 0

Cold stress and recovery in shoots and roots of Oryza sativa

ABSTRACT: Proteomics analyses of roots and shoots of O. sativa plants exposed to cold stress.

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Oryza Sativa (rice)

TISSUE(S): Root, Shoot

SUBMITTER: Martin Cerny

LAB HEAD: Martin Cerny

PROVIDER: PXD028712 | Pride | 2022-02-16

REPOSITORIES: Pride

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Dataset's files

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			Action	DRS
	Oryza_sativa_A01.msf	Msf
	Oryza_sativa_A01.raw	Raw
	Oryza_sativa_A02.msf	Msf
	Oryza_sativa_A02.raw	Raw
	Oryza_sativa_A03.msf	Msf

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Publications

The Omics Hunt for Novel Molecular Markers of Resistance to Phytophthora infestans.

Dufková Hana H Berka Miroslav M Greplová Marie M Shejbalová Šarlota Š Hampejsová Romana R Luklová Markéta M Domkářová Jaroslava J Novák Jan J Kopačka Viktor V Brzobohatý Břetislav B Černý Martin M

Plants (Basel, Switzerland) 20211225 1

Wild Solanum accessions are a treasured source of resistance against pathogens, including oomycete Phytophthora infestans, causing late blight disease. Here, Solanum pinnatisectum, Solanum tuberosum, and the somatic hybrid between these two lines were analyzed, representing resistant, susceptible, and moderately resistant genotypes, respectively. Proteome and metabolome analyses showed that the infection had the highest impact on leaves of the resistant plant and indi ...[more]

PMID: 35009065

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Project description:Escherichia coli (ATCC 25922) was cultured with sub-inhibitory doses of ZnO and ZnO nanoparticles for up to 40 subculture cycles, and the effect on the bacteria proteome was analyzed.

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Comparative transcriptomic analysis of iron deficiency response in roots of Oryza sativa and O. rufipogon

Project description:Iron (Fe) is an essential micronutrient for almost all organisms. However, Fe is frequently inaccessible to plants. To acquire insoluble Fe from soil, rice (Oryza sativa L.) plants employ a Combined Strategy, which is composed by all features of Strategy II, common to all Poaceae species, and some features of the Strategy I from non-Poaceae. To understand the evolution of Fe uptake mechanisms, we analyzed the root transcriptomic response to Fe deficiency in two species from the Oryza genus: O. sativa and its wild progenitor O. rufipogon. A total of 622 and 2,017 genes were found to be differentially expressed in O. sativa and O. rufipogon, respectively. Among the genes regulated in both species, we found the Fe transporters associated with Strategy I, such as IRT1, IRT2 and NRAMP1; and genes associated with Strategy II, such as YSL15 and IRO2.

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Serial-Omics of Equine Urine - Serial-omics characterization of equine urine

Project description:Horse urine is easily collected and contains molecules readily measurable using mass spectrometry that can be used as biomarkers representative of health, disease or drug tampering. This study aimed at analyzing microliter levels of horse urine to purify, identify and quantify proteins, polar metabolites and non-polar lipids. Urine from a healthy 12 year old quarter horse mare on a diet of grass hay and vitamin/mineral supplements with limited pasture access was collected for serial-omics characterization. The urine was treated with methyl tert-butyl ether (MTBE) and methanol to partition into three distinct layers for protein, non-polar lipid and polar metabolite content from a single liquid-liquid extraction and was repeated two times. Each layer was analyzed by high performance liquid chromatography – high resolution tandem mass spectrometry (LC-MS/MS) to obtain protein sequence and relative protein levels as well as identify and quantify small polar metabolites and lipids. The results show 46 urine proteins, many related to normal kidney function, structural and circulatory proteins as well as 474 small polar metabolites but only 10 lipid molecules. Metabolites were mostly related to urea cycle and ammonia recycling as well as amino acid related pathways, plant diet specific molecules, etc. The few lipids represented triglycerides and phospholipids. These data show a complete mass spectrometry based –omics characterization of equine urine from a single 333 uL mid-stream urine aliquot. The data can be used as a baseline for healthy urine composition and analyses can be used to monitor disease progression, health status, monitor drug use, etc.

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Endophytic fungus Acremonium alternatum modulates redox homeostasis through hormone-driven ROS signaling, leading to enhanced salt stress tolerance in Brassica napus

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Silicon effect on suberization and lignification in roots of rice (Oryza sativa)

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Proteomic analysis of sap reveals root-shoot communication dynamics in potato and barley following flg22 treatment

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