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Card19 Iinteraction Partners in Murine Macrophages

ABSTRACT: The function of the mitochondrial protein CARD19 remains largely unknown. To begin to understand the molecular function of CARD19, we performed mass spectrometry-based proteomics to identify interacting partners of CARD19 in murine macrophages. We analyzed anti-MYC tag immunoprecipitates of 3xMYC-CARD19, expressed at approximately endogenous levels in raf-myc-immortalizd macrophages from a Card19-/- strain (Jackson Labs stock # 036463).

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Blood, Macrophage

SUBMITTER: Thomas Conrads

LAB HEAD: Brian C. Schaefer

PROVIDER: PXD029157 | Pride | 2022-05-19

REPOSITORIES: Pride

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			Action	DRS
	Card19_Myc-IP_Sample_Reference.xlsx	Xlsx
	Mm_Myc_SP_082216.fasta	Fasta
	No1_3xMyc_resin_01.raw	Raw
	No1_3xMyc_resin_01_PSMs.txt	Txt
	No1_3xMyc_resin_02.raw	Raw

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Publications

CARD19 Interacts with Mitochondrial Contact Site and Cristae Organizing System Constituent Proteins and Regulates Cristae Morphology.

Rios Kariana E KE Zhou Ming M Lott Nathaniel M NM Beauregard Chelsi R CR McDaniel Dennis P DP Conrads Thomas P TP Schaefer Brian C BC

Cells 20220331 7

CARD19 is a mitochondrial protein of unknown function. While CARD19 was originally reported to regulate TCR-dependent NF-κB activation via interaction with BCL10, this function is not recapitulated ex vivo in primary murine CD8<sup>+</sup> T cells. Here, we employ a combination of SIM, TEM, and confocal microscopy, along with proteinase K protection assays and proteomics approaches, to identify interacting partners of CARD19 in macrophages. Our data show that CARD19 is specifically localized to ...[more]

PMID: 35406738

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