Dataset Information

Proteomics analysis on cottonseed meal protein isolate extract-ed using improved gossypol removal method

ABSTRACT: Cottonseed meal (CSM) contains a high amount of underutilised proteins due to the presence of gossypol, a toxic compound unsuitable for human consumption. We designed a protein extract process to generate CSM protein isolate (CSMPI) with ultra-low gossypol content to unlock their potential. This process utilised alkaline-based protein extraction with gossypol removal through a multistep acidified organic solvent treatment. Overall, our method successfully yielded 22% of CSMPI from CSM and achieved a protein purity of 92.6%. The method has reduced the free and total gossypol content of the post-treated CSMPI to 4.8ppm and 147.2ppm, respectively, which is much lower than the limit set by US FDA. Proteomics analysis indicated that the gossypol re-moval treatment increased protein identification and enhanced the nutritional quality of the CSMPI due to increased branched-chain amino acids (BCAAs) content after treatment. Overall, our results help to navigate and direct the application of CSMPI towards nutritive human food application.

INSTRUMENT(S): TripleTOF 6600

ORGANISM(S): Gossypium Hirsutum

SUBMITTER: Soon Hong Kwan

LAB HEAD: Bi Xuezhi

PROVIDER: PXD029330 | Pride | 2022-10-13

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	CSM_PostTreatment_Replicate1.mgf	Mgf
	CSM_PostTreatment_Replicate1.p	Other
	CSM_PostTreatment_Replicate1.wiff	Wiff
	CSM_PostTreatment_Replicate1.wiff.scan	Wiff
	CSM_PostTreatment_Replicate2.mgf	Mgf

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Publications

Cottonseed Meal Protein Isolate as a New Source of Alternative Proteins: A Proteomics Perspective.

Tan Chee Fan CF Kwan Soon Hong SH Lee Chun Shing CS Soh Yan Ni Annie YNA Ho Ying Swan YS Bi Xuezhi X

International journal of molecular sciences 20220903 17

Cottonseed meal (CSM) is a good source of dietary proteins but is unsuitable for human consumption due to its gossypol content. To unlock its potential, we developed a protein extraction process with a gossypol removal treatment to generate CSM protein isolate (CSMPI) with ultra-low gossypol content. This process successfully reduced the free and total gossypol content to 4.8 ppm and 147.2 ppm, respectively, far below the US FDA limit. In addition, the functional characterisation of CSMPI reveal ...[more]

PMID: 36077502

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