Proteomics

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Identification of Yap-interacting proteins in Xenopus egg extracts by co-immunoprecipitation coupled to LC/MS/MS

ABSTRACT: Yap (Yes-associated protein 1) is a downstream effector of the Hippo signaling pathway and known as a transcriptional co-activator. We previously identified Yap1 as a novel factor implicated in the control of the temporal DNA replication program in Xenopus retinal stem cells. However, whether Yap is directly involved in DNA replication dynamics and whether it could regulate DNA replication during early embryonic divisions in the absence of transcription remained to be investigated. We now found that in Yap depleted Xenopus laevis egg extracts the activation of replication origins was increased. In order to understand by which mechanism Yap could regulate the initiation of DNA replication in the absence of transcription we searched for new interacting factors. We immunoprecipitated Yap from Xenopus laevis egg extracts using anti-Yap, compared to control antibodies, and analyzed co-immunoprecipitating proteins by nanoLC/MS/MS.

INSTRUMENT(S): TripleTOF 4600

ORGANISM(S): Xenopus Laevis (african Clawed Frog)

TISSUE(S): Egg

SUBMITTER: David Cornu  

LAB HEAD: David Cornu

PROVIDER: PXD029345 | Pride | 2022-08-09

REPOSITORIES: Pride

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Dataset's files

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Action DRS
Melendez__Garcia-Co_IP_YAP.xlsx Xlsx
Rodrigo_CTRL_230817.wiff Wiff
Rodrigo_CTRL_230817.wiff.scan Wiff
Rodrigo_CTRL_230817__F067492_.mzid.gz Mzid
Rodrigo_CTRL_230817__F067492_.mzid_Rodrigo_CTRL_230817__F067492_.MGF Mzid
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Publications

A non-transcriptional function of Yap regulates the DNA replication program in <i>Xenopus laevis</i>.

Meléndez García Rodrigo R   Haccard Olivier O   Chesneau Albert A   Narassimprakash Hemalatha H   Roger Jérôme J   Perron Muriel M   Marheineke Kathrin K   Bronchain Odile O  

eLife 20220715

In multicellular eukaryotic organisms, the initiation of DNA replication occurs asynchronously throughout S-phase according to a regulated replication timing program. Here, using <i>Xenopus</i> egg extracts, we showed that Yap (Yes-associated protein 1), a downstream effector of the Hippo signalling pathway, is required for the control of DNA replication dynamics. We found that Yap is recruited to chromatin at the start of DNA replication and identified Rif1, a major regulator of the DNA replica  ...[more]

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