Dataset Information

Comparative analysis of PRE DNA-binding factors interactomes: purification of Combgap, Zeste, Psq and Adf1 associated proteins

ABSTRACT: The Polycomb group (PcG) and Trithorax group (TrxG) proteins are key epigenetic regulators controlling silenced and active states of genes in multicellular organisms, respectively. In Drosophila PcG/TrxG proteins are recruited to chromatin via binding to specialized DNA-elements termed Polycomb Response Elements (PREs). While precise mechanisms of PcG/TrxG proteins recruitment remains unknown, the important role is suggested to belong to sequence specific DNA-binding factors. Here, using affinity purification coupled with high throughput mass spectrometry (IP/MS), we isolated factors associated with Combgap, Psq, Zeste and Adf1 PRE DNA-binding Drosophila proteins. As a control we used unspecific IgG. For affinity purification the nuclear extract from Drosophila S2 cells and polyclonal antibodies against Combgap, Psq, Zeste and Adf1 were used.

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Drosophila Melanogaster (fruit Fly)

TISSUE(S): Cell Culture, Sublaminar Layer S2

SUBMITTER: Rustam Ziganshin

LAB HEAD: Maksim Erokhin

PROVIDER: PXD029459 | Pride | 2024-05-22

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	20200721_003_RZ_ME_20.mgf	Mgf
	20200721_003_RZ_ME_20.raw	Raw
	20200721_006_RZ_ME_23.mgf	Mgf
	20200721_006_RZ_ME_23.raw	Raw
	20200721_007_RZ_ME_24.mgf	Mgf

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Comparative interactome analysis of the PRE DNA-binding factors: purification of the Combgap-, Zeste-, Psq-, and Adf1-associated proteins.

Chetverina Darya D Vorobyeva Nadezhda E NE Mazina Marina Yu MY Fab Lika V LV Lomaev Dmitry D Golovnina Alexandra A Mogila Vladic V Georgiev Pavel P Ziganshin Rustam H RH Erokhin Maksim M

Cellular and molecular life sciences : CMLS 20220609 7

The Polycomb group (PcG) and Trithorax group (TrxG) proteins are key epigenetic regulators controlling the silenced and active states of genes in multicellular organisms, respectively. In Drosophila, PcG/TrxG proteins are recruited to the chromatin via binding to specific DNA sequences termed polycomb response elements (PREs). While precise mechanisms of the PcG/TrxG protein recruitment remain unknown, the important role is suggested to belong to sequence-specific DNA-binding factors. At the sam ...[more]

PMID: 35676368

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