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Chromatin immunoprecipitation of three PcG proteins and of histone H3 trimethylated at Lys 27 in Drosophila

ABSTRACT: We determined the distribution of three PcG proteins: PC, PSC and E(Z), and of histone H3 trimethylated at Lys 27 by chromatin immunoprecipitation (ChIP) coupled with analysis of immunoprecipitated DNA with a high density genomic tiling microarray (Affymetrix).

ORGANISM(S): Drosophila melanogaster

SUBMITTER: Yuri Schwartz

PROVIDER: E-MEXP-535 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Genome-wide analysis of Polycomb targets in Drosophila melanogaster.

Schwartz Yuri B YB Kahn Tatyana G TG Nix David A DA Li Xiao-Yong XY Bourgon Richard R Biggin Mark M Pirrotta Vincenzo V

Nature genetics 20060528 6

Polycomb group (PcG) complexes are multiprotein assemblages that bind to chromatin and establish chromatin states leading to epigenetic silencing. PcG proteins regulate homeotic genes in flies and vertebrates, but little is known about other PcG targets and the role of the PcG in development, differentiation and disease. Here, we determined the distribution of the PcG proteins PC, E(Z) and PSC and of trimethylation of histone H3 Lys27 (me3K27) in the D. melanogaster genome. At more than 200 PcG ...[more]

PMID: 16732288

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