Proteomics

Dataset Information

0

A novel structure of GPI anchored proteins-NT5E

ABSTRACT: GPI anchors many proteins to the cell surface. GPI precursor has three mannoses, all of which are modified by ethanolamine-phosphate (EthN-P). It has been believed that EthN-P on the third mannose is always used as a bridge to the protein and EthN-P on the second mannose is removed after GPI is attached to the protein. In fact, several GPI-anchored proteins are not appreciably reduced on cells defective in PIGG, which transfers EthN-P to the second mannose. Nevertheless, mutations in PIGG cause neuronal abnormalities. Here, we show that EthN-P on the second mannose is used as a preferential bridge for several GPI-anchored proteins. Our data modifies the current view of GPI anchors and provides mechanistic basis of PIGG deficiencies.

INSTRUMENT(S): LTQ Orbitrap Velos

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Permanent Cell Line Cell, Cell Culture

SUBMITTER: Yoshiko Murakami  

LAB HEAD: Taroh Kinoshita

PROVIDER: PXD029850 | Pride | 2022-05-31

REPOSITORIES: Pride

Json Xml

Dataset's files

Source:
Action DRS
210224_Murakami_NT5E_trp_Hilic_HilicChip_180919_Loop_01.raw Raw
F074548.dat Other
F074548.mgf Mgf
F074548.mzid.gz Mzid
F074548.xml Xml
Items per page:
1 - 5 of 5
altmetric image

Publications

Ethanolamine-phosphate on the second mannose is a preferential bridge for some GPI-anchored proteins.

Ishida Mizuki M   Maki Yuta Y   Ninomiya Akinori A   Takada Yoko Y   Campeau Philippe P   Kinoshita Taroh T   Murakami Yoshiko Y  

EMBO reports 20220523 7

Glycosylphosphatidylinositols (GPIs) are glycolipids that anchor many proteins (GPI-APs) on the cell surface. The core glycan of GPI precursor has three mannoses, which in mammals, are all modified by ethanolamine-phosphate (EthN-P). It is postulated that EthN-P on the third mannose (EthN-P-Man3) is the bridge between GPI and the protein and the second (EthN-P-Man2) is removed after GPI-protein attachment. However, EthN-P-Man2 may not be always transient, as mutations of PIGG, the enzyme that tr  ...[more]

Similar Datasets

Proteomics A novel structure of GPI anchored proteins NTNG2
2022-05-31 | PXD029851 | Pride
Proteomics A novel structure of GPI anchored proteins
2022-05-31 | PXD021996 | Pride
Proteomics A novel structure of GPI anchored proteins
2022-05-31 | PXD022032 | Pride
Proteomics GPI-glycan structures of Prion from WT mouse brains
2022-04-04 | PXD029190 | Pride
Proteomics Determination of GPI structure of human CD59 in HEK293 cells
2020-02-17 | PXD014226 | Pride
Proteomics GPI-glycan structures of human Prion from a human brain
2020-04-22 | PXD017656 | Pride
Proteomics GPI-glycan structures of Prion from PGAP4-KO mouse brains
2022-04-04 | PXD029189 | Pride
Proteomics Determination of GPI structure of human CD59 in various KO CHO cells
2018-01-30 | PXD008230 | Pride
Proteomics GPI-glycan structures of human Prion from knock-in mice_LC-MSMS
2020-04-22 | PXD017655 | Pride
Proteomics GPI-glycan structures of human Prion (M232R) from knock-in mice_LC-MSMS
2023-03-08 | PXD037584 | Pride