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GPI-glycan structures of human Prion (M232R) from knock-in mice_LC-MSMS

ABSTRACT: To determine glycosylphosphatidylinositol (GPI) structures of human prion proteins with M232R mutation isolated from knock-in mice those express human prion protein instead of mouse one.

INSTRUMENT(S): LTQ Orbitrap Velos

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Brain

SUBMITTER: Tetsuya Hirata

LAB HEAD: Taroh Kinoshita

PROVIDER: PXD037584 | Pride | 2023-03-08

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	181019_Prion_trp_M233R_Hilic_HilicTip_8u1.raw	Raw
	F079187.mgf	Mgf
	F079187_M232R.dat	Other
	F079187_M232R.mzid.gz	Mzid
	checksum.txt	Txt

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Publications

A point mutation in GPI-attachment signal peptide accelerates the development of prion disease.

Kobayashi Atsushi A Kobayashi Atsushi A Hirata Tetsuya T Shimazaki Taishi T Munesue Yoshiko Y Aoshima Keisuke K Kimura Takashi T Nio-Kobayashi Junko J Hasebe Rie R Takeuchi Atsuko A Matsuura Yuichi Y Kusumi Satoshi S Koga Daisuke D Iwasaki Yasushi Y Kinoshita Taroh T Mohri Shirou S Kitamoto Tetsuyuki T

Acta neuropathologica 20230306 5

A missense variant from methionine to arginine at codon 232 (M232R) of the prion protein gene accounts for ~ 15% of Japanese patients with genetic prion diseases. However, pathogenic roles of the M232R substitution for the induction of prion disease have remained elusive because family history is usually absent in patients with M232R. In addition, the clinicopathologic phenotypes of patients with M232R are indistinguishable from those of sporadic Creutzfeldt-Jakob disease patients. Furthermore, ...[more]

PMID: 36879070

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GPI-glycan structures of human Prion from knock-in mice_LC-MSMS

Project description:To determine glycosylphosphatidylinositol (GPI) structures of human prion proteins isolated from knock-in mice those express human prion protein instead of mouse one.

2020-04-22 | PXD017655 | Pride

GPI-glycan structures of human Prion from a human brain

Project description:To determine glycosylphosphatidylinositol (GPI) structures of human prion proteins isolated from a human brain.

2020-04-22 | PXD017656 | Pride

GPI-glycan structures of Prion from WT mouse brains

Project description:To determine glycosylphosphatidylinositol (GPI) structures of prion proteins isolated from mouse brains.

2022-04-04 | PXD029190 | Pride

GPI-glycan structures of Prion from PGAP4-KO mouse brains

Project description:To determine glycosylphosphatidylinositol (GPI) structures of prion proteins isolated from PGAP4-KO mouse brains.

2022-04-04 | PXD029189 | Pride

Determination of GPI structure of human CD59 in HEK293 cells

Project description:To determine GPI structure of human CD59 in wild-type,B3GALT4-KO, PIGZ-B3GALT4-DKO HEK293 cells.

2020-02-17 | PXD014226 | Pride

A novel structure of GPI anchored proteins-NT5E

Project description:GPI anchors many proteins to the cell surface. GPI precursor has three mannoses, all of which are modified by ethanolamine-phosphate (EthN-P). It has been believed that EthN-P on the third mannose is always used as a bridge to the protein and EthN-P on the second mannose is removed after GPI is attached to the protein. In fact, several GPI-anchored proteins are not appreciably reduced on cells defective in PIGG, which transfers EthN-P to the second mannose. Nevertheless, mutations in PIGG cause neuronal abnormalities. Here, we show that EthN-P on the second mannose is used as a preferential bridge for several GPI-anchored proteins. Our data modifies the current view of GPI anchors and provides mechanistic basis of PIGG deficiencies.

2022-05-31 | PXD029850 | Pride

A novel structure of GPI anchored proteins NTNG2

2022-05-31 | PXD029851 | Pride

Efficacy and molecular mechanism of a glycoside compound inhibiting abnormal prion protein formation in prion-infected cells

Project description:This report describes our study of the efficacy and the potential mechanism underlying the anti-prion action of a new anti-prion compound having a glycoside structure in prion-infected cells. The study revealed involvements of two factors in the mechanism of the compound action: interferon and a microtubule nucleation activator, phosphodiesterase 4D interacting protein. In particular, phosphodiesterase 4D interacting protein was suggested to be important in regulating the trafficking or fusion of prion protein-containing vesicles or structures in cells. The findings of the study are expected to be useful not only for the elucidation of cellular regulatory mechanisms of prion protein, but also for the implication of new targets for therapeutic development. Prion-infected N167 cells were treated with either anti-prion glycoside compound (Gly-9) or control glycoside compound (Gly-14) at a dose of 5 M-NM-<g/mL for three days. Then, gene expression profiles were analyzed by DNA microarray analysis. Experiments were performed in quadruplicate.

2014-02-22 | E-GEOD-55241 | biostudies-arrayexpress

A novel structure of GPI anchored proteins

Project description:GPI anchors many proteins to the cell surface. GPI precursor has three mannoses, all of which are modified by ethanolamine-phosphate (EthN-P). It has been believed that EthN-P on the third mannose is always used as a bridge to the protein and EthN-P on the second mannose is removed after GPI is attached to the protein. Based on the finding that PIGB-knockout cells lacking the third mannose and PIGO-knockout cells lacking EthN-P linked to the third mannose expressed low levels of CD59 and DAF, GPI-APs and that those residual GPI-APs were lost by further knockout of PIGG. Using mass-spectrometry we determined that CD59 expressed on PIGB-knockout cells is attached to GPI via EthN-P linked to the second mannose. This linkage structure is also found in wild type cells. Our data modifies the current view of GPI anchors and provides mechanistic basis of inherited GPI deficiency caused by PIGG mutations.

2022-05-31 | PXD021996 | Pride

Gene expression profile of a murin neuroblastoma cell line infected with the prion strain 22L

Project description:The underlying pathogenic mechanisms of prion infection are not well characterized. To study the effect of prion infection on gene expression in neuronal cell cultures, a neuroblastoma (N2a) cell clone was infected with either the mouse adapted prion strain 22L or exposed to uninfected brain homogenate as a negative control. Large scale expression analysis was performed using a cDNA microarray chip comprising about 21,000 spotted ESTs. Over hundred genes were identified that are differentially expressed in 22L-infected cells when compared to uninfected cells. Several of the identified changes in gene expression have also been reported for other neurodegenerative diseases such as Alzheimer`s disease. Keywords: cDNA arrays, prion, N2a, neuroblastoma cell line, murine A neuroblastoma (N2a) cell clone was infected with either the mouse adapted prion strain 22L or exposed to uninfected brain homogenate as a negative control. Eight replicates including four dye swap experiments have been performed for the comparison of prion infected cells versus control cells.

2010-05-26 | E-GEOD-7119 | biostudies-arrayexpress

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