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Suppressors of amyloid-beta toxicity improve recombinant protein production by reducing oxidative stress and tunning cellular metabolism

ABSTRACT: In this study, we used a yeast Alzheimer’s disease model in which amyloid-β peptides (Aβ42) accumulate and induce stress-related phenotypes similar to overexpression of recombinant proteins. We validated that suppressors of Aβ42 cytotoxicity could reduce cell stress and improve recombinant protein production. Omics analyses and reverse metabolic engineering reveal potential regulatory hubs in cellular metabolism and protein synthesis, which may provide guidelines for engineering other cell factories for efficient protein production.

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Saccharomyces Cerevisiae (baker's Yeast)

SUBMITTER: Egor Vorontsov

LAB HEAD: Martin Engqvist

PROVIDER: PXD030111 | Pride | 2022-05-12

REPOSITORIES: pride

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			Action	DRS
	4124_ProteomeDiscoverere24.msf	Msf
	4124_ProteomeDiscoverere24.pdResult	Other
	4124_TMTpro_Output_Tables.zip	Other
	Labeling_Scheme.pdf	Pdf
	Lumos_201221_04.raw	Raw

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