Dataset Information

Interactome Analysis of NDP52 by LC-MS/MS

ABSTRACT: NDP52 is an autophagy receptor, with known cytoplasmic binding partners. However, little is known regarding the nuclear functions of this protein. To investigate possible nuclear roles of NDP52 and find new interacting proteins, we performed LC-MS/MS affinity purification experiments.

INSTRUMENT(S): Synapt MS

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Permanent Cell Line Cell, Hela Cell

DISEASE(S): Cervix Carcinoma

SUBMITTER: Chris Toseland

LAB HEAD: Christopher Toseland

PROVIDER: PXD030238 | Pride | 2023-03-29

REPOSITORIES: Pride

ACCESS DATA

Dataset's files

Source:

			Action	DRS
	20190916_NDP52_01.raw.zip	Raw
	20190916_NDP52_01_IA_final_fragment_raw_1.csv	Csv
	20190916_NDP52_02.raw.zip	Raw
	20190916_NDP52_02_IA_final_fragment_raw_2.csv	Csv
	20190916_NDP52_03.raw.zip	Raw

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Publications

Autophagy receptor NDP52 alters DNA conformation to modulate RNA polymerase II transcription.

Dos Santos Ália Á Rollins Daniel E DE Hari-Gupta Yukti Y McArthur Hannah H Du Mingxue M Ru Sabrina Yong Zi SYZ Pidlisna Kseniia K Stranger Ane A Lorgat Faeeza F Lambert Danielle D Brown Ian I Howland Kevin K Aaron Jesse J Wang Lin L Ellis Peter J I PJI Chew Teng-Leong TL Martin-Fernandez Marisa M Pyne Alice L B ALB Toseland Christopher P CP

Nature communications 20230518 1

NDP52 is an autophagy receptor involved in the recognition and degradation of invading pathogens and damaged organelles. Although NDP52 was first identified in the nucleus and is expressed throughout the cell, to date, there is no clear nuclear functions for NDP52. Here, we use a multidisciplinary approach to characterise the biochemical properties and nuclear roles of NDP52. We find that NDP52 clusters with RNA Polymerase II (RNAPII) at transcription initiation sites and that its overexpression ...[more]

PMID: 37202403

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