Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

Transcription profiling of C. elegans strains IG274 wt;frIs7 and CZ5701 dapk-1(ju4);frIs7 infected with Dreshmeria coniosporia

ABSTRACT: 1.Sample Growth Conditions protocols
Eggs from IG274 wt; frIs7 and dapk-1(ju4); frIs7 worms cultivated at 25°C were allowed to hatch in the absence of food at 25°C for 16 hours. Synchronised larvae were transferred to NGM agar plates lawned with OP50 and cultivated at 25°C for 48 hours.
2.Sample Treatment protocols
Synchronised IG274 wt; frIs7 and CZ5701 dapk-1(ju4); frIs7 worms at the young L4 stage were infected with freshly harvested spores of Dreshmeria coniosporia. After 24 hours at 25°C, the worms were collected into trizol for further RNA extraction. Controls worms were non infected.

INSTRUMENT(S): ScanArray 4000XL [PerkinElmer]

ORGANISM(S): Caenorhabditis elegans

DISEASE(S): not infected

SUBMITTER: Jonathan Ewbank

PROVIDER: E-MEXP-1363 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Negative regulation of Caenorhabditis elegans epidermal damage responses by death-associated protein kinase.

Tong Amy A Lynn Grace G Ngo Vy V Wong Daniel D Moseley Sarah L SL Ewbank Jonathan J JJ Goncharov Alexandr A Wu Yi-Chun YC Pujol Nathalie N Chisholm Andrew D AD

Proceedings of the National Academy of Sciences of the United States of America 20090121 5

Wounding of epidermal layers triggers multiple coordinated responses to damage. We show here that the Caenorhabditis elegans ortholog of the tumor suppressor death-associated protein kinase, dapk-1, acts as a previously undescribed negative regulator of barrier repair and innate immune responses to wounding. Loss of DAPK-1 function results in constitutive formation of scar-like structures in the cuticle, and up-regulation of innate immune responses to damage. Overexpression of DAPK-1 represses i ...[more]

PMID: 19164535

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Project description:Filarial nematodes are arthropod-borne nematodes that cause a variety of economically important diseases such as onchocerciasis (river blindness), lymphatic filariasis, and heartworm disease. The most widespread filarial disease of humans is lymphatic filariasis, caused by worms in the genera Wuchereria and Brugia. Lymphatic filariasis is an economic and social burden in endemic countries and affects approximately 119 million people worldwide (Michael, 1997). In humans, the worms live in and block the lymph vessels, causing improper flow of lymph, and inflammation of the lymphatic system. The symptoms are fever, swollen limbs and genitals, generalized malaise, and can progress to a debilitating condition known as elephantiasis This research focuses on the transmission of these worms to the disseminating mosquito host, and it is based on the interesting observation that mf must be at least 7 days old to successfully infect the mosquito (de Hollanda, 1982). Newborn mf that have not â??maturedâ?? cannot successfully penetrate the midgut of the mosquito, and subsequently cannot develop to the L3 stage (Fuhrman, 1987). Previous work done by another group 20 years ago suggests that the molecular makeup of the worm surface changes during this maturation process (Furman, 1983 a and b). We used microarray analysis to characterize changes in gene expression that take place during the mf maturation process. Understanding the gene expression changes that occur as the mf mature will allow us to understand the nature of the philological transition that allows mf to move from the human to the mosquito host. With this information in hand, we can eventually identify parasite molecules that could be targeted to either stop parasite reproduction or prevent transmission of the mf to the mosquito. This would stop parasite transmission in endemic areas. Brugia pahangi mature mf (30 days and older) RNA was compared to immature mf (3 days and younger). Three biological replicates were performed each with two technical replicates

Dataset Information

Transcription profiling of C. elegans strains IG274 wt;frIs7 and CZ5701 dapk-1(ju4);frIs7 infected with Dreshmeria coniosporia

Publications

Negative regulation of Caenorhabditis elegans epidermal damage responses by death-associated protein kinase.

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