Proteomics

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Proteomics and phosphoproteomics analysis of the vitreous in idiopathic epiretinal membrane patients


ABSTRACT: Purpose: The purpose of present study is to characterize the Idiopathic epiretinal membrane (iERM) through phosphoproteomics to facilitate its diagnosis and treatment. Experimental design: The vitreous of 25 patients with iERM and 15 patients with idiopathic macular hole were analyzed by proteomic and phosphoproteomic analysis based on tandem mass tag. PRM was used to verify differential proteins. Results: Proteomic analysis identified a total of 878 proteins, including 50 differential proteins. Phosphoproteomic analysis identified a total of 401 phosphorylation sites on 213 proteins, including 27 differential phosphorylation sites on 24 proteins. Twenty six proteins of these differential proteins and phosphorylated proteins may be closely related to fibrosis in iERM. MAPKAPK3 and MAPKAPK5 are predicted as the major kinases in vitreous of iERM. Conclusion and clinical relevance: Our results screened candidate proteins for the treatment of fibrosis in iERM, among which tenascin-C, galectin-3-binding protein, glucose-6-phosphate isomerase, neuroserpin, collagen alpha-1(XI) chain and collagen alpha-1(II) chain are expected to become potential biomarkers or therapeutic targets for iERM. In addition, this article expand the data of vitreous phosphoproteome, and provide a reference resource and new enlightenment for studying the pathogenic mechanisms of retinal diseases.

INSTRUMENT(S): Orbitrap Exploris 480

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Eye

SUBMITTER: Chao Sun  

LAB HEAD: chao sun

PROVIDER: PXD030278 | Pride | 2023-12-04

REPOSITORIES: Pride

Dataset's files

Source:
Action DRS
HA067PRM1_iERM_VH_1.zip Other
HA067PRM1_iERM_VH_2.zip Other
HA067PRM1_iERM_VH_3.zip Other
HA067PRM1_iERM_VH_4.zip Other
HA067PRM1_iERM_VH_5.zip Other
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Publications

Proteomics and phosphoproteomics analysis of vitreous in idiopathic epiretinal membrane patients.

Sun Chao C   Zou Huan H   Yang Zhouquan Z   Yang Mei M   Chen Xiaofan X   Huang Yanming Y   Fan Wei W   Yuan Rongdi R  

Proteomics. Clinical applications 20220511 5


<h4>Purpose</h4>The purpose of the present study was to characterize the idiopathic epiretinal membrane (iERM) through proteomics and phosphoproteomics analysis to facilitate the diagnosis and treatment of iERM.<h4>Experimental design</h4>The vitreous of 25 patients with an iERM and 15 patients with an idiopathic macular hole were analyzed by proteomic and phosphoproteomic analysis based on tandem mass tag. PRM was used to verify the differential proteins.<h4>Results</h4>Proteomic analysis ident  ...[more]

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