Proteomics

Dataset Information

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DNR pulse treatment of primary AML


ABSTRACT: Primary human AML bone marrow sample (newly diagnosed, prior to treatment initation) was obtained from donor after consent and AML blasts were isolated by standard Ficoll centrifugation. AML blasts were pulse-treated with daunorubicine (100 nM, 200 nM, 400 nM) or DMSO control for 1 hour, followed by wash-out and short-term cell culture to allow cellular responses to develop. 20,000 cells were analysed by LC-MS3, and 5,000 cells were subjected to flow cytometry apoptosis assay.

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Leukocyte, Blood

DISEASE(S): Acute Leukemia

SUBMITTER: Sebastian Koschade  

LAB HEAD: Christian Muench

PROVIDER: PXD030280 | Pride | 2022-10-15

REPOSITORIES: Pride

Dataset's files

Source:
Action DRS
20210422_MAA_SK_3_103_8F.msf Msf
20210422_MAA_SK_3_103_8F_F1.raw Raw
20210422_MAA_SK_3_103_8F_F2.raw Raw
20210422_MAA_SK_3_103_8F_F3.raw Raw
20210422_MAA_SK_3_103_8F_F4.raw Raw
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