Fasciola hepatica cathepsin L zymogens: immuno-proteomic evidence for highly immunogenic zymogen-specific conformational epitopes to support diagnostics development
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ABSTRACT: We conducted immuno-proteomic investigations of recombinant and native procathepsins ((r)FhpCL1) and counterpart polyclonal antibodies to a recombinant mutant procathepsin L (anti-rFhΔpCL1). Proteomics including gel electrophoresis and liquid chromatography tandem mass spectrometry were used for formal identification and in the determination of sample compositions. We have confirmed recombinant and native cathepsin L zymogens contain conserved, highly antigenic epitopes that are conformationally dependent.
INSTRUMENT(S): 6550 iFunnel Q-TOF LC/MS, Orbitrap Fusion
ORGANISM(S): Fasciola Hepatica (liver Fluke)
DISEASE(S): Fascioliasis
SUBMITTER: Clare Collett
LAB HEAD: Professor Peter Brophy
PROVIDER: PXD030293 | Pride | 2022-07-19
REPOSITORIES: Pride
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