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Investigating the transition of Apis mellifera summer workers to diutinus workers using proteomic techniques.

ABSTRACT: Apis mellifera workers in temperate climates display two castes; short lived summer bees that engage in nursing, hive maintenance and foraging, and long lived winter bees (diutinus bees) which remain within the hive and are essential for thermoregulation. Label free quantitative proteomic analysis was conducted on A. mellifera workers sampled from July to October 2019 to compare the proteomes of workers as the colony progresses through the year. Proteomic analysis revealed a shift in protein expression in workers in September and October in comparison to July and August samples. Workers samples in September and October had a higher abundance of proteins associated with oxidative phosphorylation and storage proteins such as hexamerin. Interestingly, a shift in protein expression was detected in newly emerged bees between July to October, providing evidence that workers have adapted to emerge with a different protein profile in preparation for the winter months.

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Apis Mellifera (honeybee)

SUBMITTER: Rachel Ward

LAB HEAD: Kevin Kavanagh

PROVIDER: PXD032755 | Pride | 2022-11-21

REPOSITORIES: Pride

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Project description:This study aims to examine global gene expression profiles before and after the work-shift among coke-oven workers (COW). COW work six consecutive days and then take two days off. Two blood and urine samples in each worker were collected before starting to work after two-days off and end-of-shift in the sixth-day work in 2009. Altered gene expressions (ratio of gene expression levels between end-of-shift and pre-shift work) were performed by Human OneArray expression system which probes ~30,000-transcription expression profiling of human genes. Sixteen workers, all men, were enrolled in this study. Median urinary 1-hydroxypyrene (1OHP) levels (mole/mole creatinine) in end-of-shift work were significantly higher than those in pre-shift work (2.58 vs. 0.29, p = 0.0002). Among the 20,341 genes which passed experimental quality control, 26 gene expression changes, 7 positively- and 19 negatively-, were highly correlated with across-the-shift urinary 1OHP levels (end-of-shift – pre-shift 1OHP) (p-value < 0.001). The high and low exposure groups of across-the-shift urinary 1OHP levels dichotomized in ~2.00 µmole/mole creatinine were able to be distinguished by these 26 genes. Some of them are known to be involved in apoptosis, chromosome stability/DNA repair, cell cycle control/tumor suppressor, cell adhesion, development/spermatogenesis, immune function, and neuronal cell function. Sixteen coke-oven workers who had worked in one of two coke-oven plants for at least one year in the largest steel company in Taiwan voluntarily participated in this study between July-October, 2009. Coke-oven workers regularly work 6 days and take two days off. Thus, we collected their blood and urine samples at two different time points: one was during the pre-shift work on the first day after two days off, and the second one was at the end-of-shift work on the 6th work day. Information about age and smoking status was also collected before the collection of biological specimens.

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Investigating the transition of Apis mellifera summer workers to diutinus workers using proteomic techniques.

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