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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Transcription profiling of abdomens from wild type and anarchistic strains of worker honey bees to identify genes related to ovary activation

ABSTRACT: This experiment examines differences in gene expression between wildtype and an experimental strain (anarchistic) of worker honey bees (Apis mellifera). Mature wildtype and anarchistic workers tend to have non-active and active ovaries, respectively. Thus young workers from these strains are expected to show differential expression at loci involved in the regulation of worker reproduction, which occurs via arrhenotokous parthenogenesis.

ORGANISM(S): Apis mellifera

SUBMITTER: Graham Thompson

PROVIDER: E-MEXP-1549 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Transcription profiling of genes related to ovary activation in worker honey bee brains

Project description:This experiment examines differences in gene expression between wildtype and an experimental strain (anarchistic) of worker honey bees (Apis mellifera). Mature wildtype and anarchistic workers tend to have non-active and active ovaries, respectively. Thus young workers from these strains are expected to show differential expression at loci involved in the regulation of worker reproduction, which occurs via arrhenotokous parthenogenesis.

2008-07-11 | E-MEXP-1552 | biostudies-arrayexpress

Transcription profiling of abdomens from anarchist and wild-type honeybee strains to investigate ovary activation

Project description:Effect of genotype (wildtype vs. anarchist) on gene expression in developing (four-day-old) honeybee worker abdomens. Goal of experiment is to identify genes associated with ovary activation during worker development. These genes are hypothesised to be candidates for the regulation of worker sterility.

2006-10-01 | E-MEXP-705 | biostudies-arrayexpress

Transcription profiling of brains from anarchist and wild-type honeybee strains to investigate ovary activation

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2006-10-01 | E-MEXP-699 | biostudies-arrayexpress

Genomics of colony founding in fire ants - pleo2

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2013-08-21 | E-MEXP-3898 | biostudies-arrayexpress

Gene expression of metamorphosis in fire ants

Project description:Ants are among the most successful animals on earth, with societies of a complexity that rivals our own. These societies are characterized by reproductive division of labor between female queens that can live several years and lay thousands of eggs per day, workers that live only a few months and are sterile, and males that live only a few weeks and do not participate in colony tasks. These striking differences in lifespan and roles are echoed by extensive morphological and physiological divergence. Using the fire ant, Solenopsis invicta, we conduct the first genome-wide survey of developmental gene expression levels over 20 time-points from larval to adult stages in workers, queens and males Three castes: Worker, queen & male; four colonies used for each caste (12 colonies total). Timepoints: 0h, 3h, 6h, 12h, 18h, 24h, 36h and 48h (hour timepoints) and once every 24hours until eclosion (day timepoints). Eclosion occured after 15 days for male (21 timepoints), 14 days for queen (20 timepoints) and 12 days for worker larvae (19 timepoints). We used a loop design with direct comparisons of consecutive samples within each cast: two loops in one Dye-direction, two loops in the other for balance. Additionally, 3-5 hybridizations against an unrelated Reference RNA were performed within each replicate loop. Microarray batch is indicated in the description column

2013-10-20 | E-GEOD-24906 | biostudies-arrayexpress

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2008-07-29 | E-MEXP-1044 | biostudies-arrayexpress

Changes in liver proteins of rats fed both low and high-fat and sucrose diets induced by fish omega-3 PUFAs and their combination with grape polyphenols according to quantitative proteomics, part 2

Project description:This study considers the physiological modulation of liver proteins due to the supplementation with fish oils under two different dietary backgrounds: low- or high- fat and sucrose diets, and the effect of their combination with an antioxidant agent (grape polyphenols) which provides reducing power. For this scope, a quantitative proteomics approach based on the Isobaric Tag for relative and Absolute Quantitation methodology (iTRAQ)-coupled to nano-LC-MS/MS and complemented with 2D-DIGE analysis were used for determining the regulation of liver proteins exerted by the supplementation with fish oils, polyphenols or their combination of Wistar Kyoto rats in the two chosen dietary backgrounds. This experimental design was useful to investigate if the behavior of fish oils changes when the dietary background is modified and the possible synergy between fish oils and polyphenols. Results show that the capacity of fish oils, polyphenols or their combination for down or up-regulating liver proteins depends on the dietary context. In the background of low-fat low-sucrose healthy diets, 10 different proteins were altered by the sum of three supplements, in opposite to the 45 altered proteins found in the high-fat high-sucrose unhealthy diets. In both situations, fish oils seemed to be the main force for regulating liver proteins, although the addition of polyphenols was able to modulate some fish oils effects. Moreover, we provide evidence of the effect of fish oils and their combination with grape polyphenols for improving biochemical parameters and for reducing enzymes of hepatic lipogenesis and glycolysis, for enhancing fatty acid beta oxidation and insulin signaling and for the amelioration of endoplasmic reticule stress and protein oxidation when are included in an unhealthy diet.

2017-01-17 | PXD003080 | Pride

Transcription profiling of yeast TOP1 null strain bearing plasmids that express wild-type yeast Top1p or CEN4-ARS1-URA3 yeast sequences

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2008-06-01 | E-MEXP-1080 | biostudies-arrayexpress

Transcription profiling of yeast TOP1 null strain bearing plasmids that express wild-type yeast Top1p or an inactive Y727F mutant enzyme and treated with camptothecin

Project description:To establish the effect of CPT in the regulation of global transcription in S. cerevisiae, we have used a yeast TOP1 null strain, JEL1Δtop1, bearing a low-copy number plasmid that expresses, under the control of the yeast TOP1 promoter, either a wild-type (wt) yeast Top1p (pCC10) or an inactive Y727F mutant enzyme (pAR7).We determined global transcript levels upon CPT treatments of JEL1Δtop1 cells expressing either a yeast wt or Y727F mutant Top1p.

2008-06-01 | E-MEXP-1104 | biostudies-arrayexpress

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