Dataset Information

Surveyance of apparent proteome unfolding curves via thiol labelling under HSP70 inhibition

ABSTRACT: Census of apparent proteome unfolding curves following urea denaturation in cell lysate derived from control of VER155008-treated N2a cells using thiol reactivity probe tetraphenylethene maleimide (TPE-MI).

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Mus Musculus (mouse)

SUBMITTER: Dezerae Cox

LAB HEAD: Danny Hatters

PROVIDER: PXD030567 | Pride | 2022-05-20

REPOSITORIES: Pride

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Publications

Hidden information on protein function in censuses of proteome foldedness.

Cox Dezerae D Ang Ching-Seng CS Nillegoda Nadinath B NB Reid Gavin E GE Hatters Danny M DM

Nature communications 20220414 1

Methods that assay protein foldedness with proteomics have generated censuses of apparent protein folding stabilities in biological milieu. However, different censuses poorly correlate with each other. Here, we show that the reason for this is that methods targeting foldedness through monitoring amino acid sidechain reactivity also detect changes in conformation and ligand binding, which can be a substantial fraction of the data. We show that the reactivity of only one quarter of cysteine or met ...[more]

PMID: 35422070

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