Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Surveyance of chaperone-client interactions via thiol labelling

ABSTRACT: Census of apparent chaperone-client interaction using thiol reactivity probe tetraphenylethene maleimide (TPE-MI).

INSTRUMENT(S): Orbitrap Eclipse

ORGANISM(S): Homo Sapiens (human) Sus Scrofa Domesticus (domestic Pig)

SUBMITTER: Dezerae Cox

LAB HEAD: Danny M. Hatters

PROVIDER: PXD022587 | Pride | 2022-05-19

REPOSITORIES: Pride

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	200706_DC295.raw	Raw
	200706_DC296.raw	Raw
	200706_DC297.raw	Raw
	200706_DC298.raw	Raw
	200706_DC299.raw	Raw

Items per page:

1 - 5 of 17

Publications

Hidden information on protein function in censuses of proteome foldedness.

Cox Dezerae D Ang Ching-Seng CS Nillegoda Nadinath B NB Reid Gavin E GE Hatters Danny M DM

Nature communications 20220414 1

Methods that assay protein foldedness with proteomics have generated censuses of apparent protein folding stabilities in biological milieu. However, different censuses poorly correlate with each other. Here, we show that the reason for this is that methods targeting foldedness through monitoring amino acid sidechain reactivity also detect changes in conformation and ligand binding, which can be a substantial fraction of the data. We show that the reactivity of only one quarter of cysteine or met ...[more]

PMID: 35422070

Similar Datasets

Surveyance of apparent proteome unfolding curves via thiol labelling

Project description:Census of apparent proteome unfolding curves following urea denaturation in cell lysate using thiol reactivity probe tetraphenylethene maleimide (TPE-MI).

2022-05-19 | PXD022640 | Pride

Surveyance of apparent proteome unfolding curves via thiol labelling under HSP70 inhibition

Project description:Census of apparent proteome unfolding curves following urea denaturation in cell lysate derived from control of VER155008-treated N2a cells using thiol reactivity probe tetraphenylethene maleimide (TPE-MI).

2022-05-20 | PXD030567 | Pride

TPE-MI reveals proteome remodelling in response to cellular stimuli

Project description:Census of apparent proteome conformation in response to stimuli using thiol reactivity probe tetraphenylethene maleimide (TPE-MI).

2023-02-04 | PXD033152 | Pride

A thiol probe for measuring unfolded protein load and proteostasis in cells

Project description:When proteostasis becomes unbalanced, unfolded proteins accumulate and can aggregate. However, probes quantifying proteostasis imbalance are lacking. We report a dye, tetraphenylethene maleimide (TPE-MI) that can measure unfolded protein load.TPE-MI fluorescence is enhanced upon reaction with cellular proteomes under conditions promoting accumulation of unfolded proteins and, via proteomic analysis, can track which proteins expose more cysteine residues under stress.

2018-10-24 | PXD006527 | Pride

affy_rose_2012_06: RhDiF1 knockdown

Project description:affy_rose_2012_06 - affy_rose_2012_06 - - Study of rose perfume by comparing a wild-tpe rose with one in which the expression of a scent gene has been altered.-Study of rose perfume by comparing a wild-tpe rose with one in which the expression of a scent gene has been altered. 8 arrays - rose; normal vs rnai mutant comparaison

2013-09-12 | E-GEOD-45236 | biostudies-arrayexpress

Molecular basis of TPE-OLD [methylation]

Project description:Among epigenetic modifiers, telomeres, represent attractive modulators of the genome in part through position effects. Telomere Position Effect – Over Long Distances (TPE-OLD) modulates genes expression by changes in telomeric-dependent long-distance loops, with a reach of 10Mb from the telomeres. However, TPE-OLD remain poorly defined. We used cells with controlled telomere length combined to a genome wide approach revealing its transcriptome and methylome. We identified a common cis element that behaved as an insulator/ enhancer. By using reporter assays integrating this element, we disclosed additional trans partners further validated by in silico data. Exploiting our cellular model we observed the depletion of one candidate (RBPJ) at TPE-OLD associated loci concomitant to telomere shortening. Therefore, we conclude that TPE-OLD is orchestrated by Alu-like elements acting as enhancers in association with RBPJ. We propose that TPE-OLD functions by the coordinated action of newly evolved enhancers; an associated protein RBPJ and telomere length to produce an adapted response to external stimuli (i.e., Aging)

2023-01-05 | GSE213427 | GEO

Molecular basis of TPE-OLD [RNA-seq]

2023-01-05 | GSE213281 | GEO

Temporal transcriptional changes in human monocytes following acute myocardial infarction: The GerMIFs monocyte expression study

Project description:Monocytes play a central role in the inflammatory response that follows acute myocardial infarction (MI). In order to study phenotypic adaptation of this cell type, we investigated patterns of monocyte gene expression in circulating monocytes at various stages of MI. Circulating monocytes were isolated from venous blood of MI patients at three time points: t1: within 6 hours after onset of chest pain (acute phase), t2: 3 days after MI (subacute phase), t3: 90 days after MI (chronic phase). For comparison, we studied a control group (n=21, data to be submitted later) with stable coronary artery disease. Using this transcriptomic analysis, we aimed to provide a more comprehensive reference of monocyte biology following acute MI and to aid in the identification of novel pathways and genes influencing the course of MI. Monocytes play a central role in the inflammatory response that follows acute myocardial infarction (MI). In order to study phenotypic adaptation of this cell type, we investigated patterns of monocyte gene expression in circulating monocytes at various stages of MI. Circulating monocytes were isolated from venous blood of MI patients at three time points: t1: within 6 hours after onset of chest pain (acute phase), t2: 3 days after MI (subacute phase), t3: 90 days after MI (chronic phase). For comparison, we studied a control group (n=21, data to be submitted later) with stable coronary artery disease. Illumina Ref-8 v3.0 microarray arrays were used for whole-genome transcriptional profiling.

2012-04-30 | E-GEOD-28454 | biostudies-arrayexpress

affy_rose_2012_06: RhDiF1 knockdown

2013-09-12 | GSE45236 | GEO

Gene Expression of Breast Cancer Cell Lines Across Biomaterial Platforms

Project description:2 Breast Cancer Cell lines were seeded onto TCPS, on 2D PEG-PC gels, and encapsulated in 3D PEG-Maleimide gels as single cells or spheroids to determine transcript level changes based on culture platform

2017-03-01 | GSE93562 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data