Proteomics

Dataset Information

0

LC-MS/MS identification of BMAL1 interaction proteins


ABSTRACT: Advances in circadian research revealed an intricate relationship between aging and circadian rhythms. However, whether and how the circadian machinery contribute to stem cell aging, especially in primates, remains poorly understood. In this study, we investigated the role of BMAL1, the only non-redundant circadian clock component, during aging in mesenchymal progenitor cells (MPCs). We observed an accelerated aging phenotype in both BMAL1 deficient human and cynomolgus monkey MPCs. Notably, this phenotype is mainly attributed to a transcriptional-independent role of BMAL1 in stabilizing the heterochromatin and thus preventing LINE1 activation. In senescent MPCs from human and cynomolgus monkeys, dampened LINE1 binding capacity of BMAL1 and synergistically activated LINE1 transcripts were observed. Furthermore, similar de-stabilized heterochromatin and aberrant LINE1s transcription was observed in the skin and muscle tissues from BMAL1-deficient cynomolgus monkey. Altogether, these findings uncover a noncanonical role of BMAL1 in stabilizing heterochromatin to inactivate LINE1 that drives aging in primates.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Cell Culture, Early Embryonic Cell

SUBMITTER: Jiang Xiaoyu  

LAB HEAD: Liang Chuqian

PROVIDER: PXD030570 | Pride | 2022-03-18

REPOSITORIES: Pride

Dataset's files

Source:
Action DRS
BMAL1.msf Msf
BMAL1.raw Raw
Luc.msf Msf
Luc.raw Raw
Items per page:
1 - 4 of 4
altmetric image

Publications


Aging in humans is intricately linked with alterations in circadian rhythms concomitant with physiological decline and stem cell exhaustion. However, whether the circadian machinery directly regulates stem cell aging, especially in primates, remains poorly understood. In this study, we found that deficiency of BMAL1, the only non-redundant circadian clock component, results in an accelerated aging phenotype in both human and cynomolgus monkey mesenchymal progenitor cells (MPCs). Unexpectedly, th  ...[more]

Similar Datasets

2024-03-05 | PXD050387 |
2020-02-11 | GSE145019 | GEO
2022-06-27 | GSE196792 | GEO
2022-06-27 | GSE196791 | GEO
2016-08-08 | E-GEOD-54436 | biostudies-arrayexpress
2024-06-22 | PXD030289 | Pride
2024-06-22 | PXD030287 | Pride
2020-06-29 | GSE146247 | GEO
2016-08-08 | GSE54436 | GEO
2023-03-01 | GSE214514 | GEO