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FastCAT accelerates absolute quantification of proteins by using multiple short non-purified chimeric standards

ABSTRACT: Absolute (molar) quantification of proteins provides the analytical rationale for system-level modelling of diverse molecular mechanisms. FastCAT method employs multiple short (<50 kDa) stable-isotope labeled chimeric proteins (CPs) composed of concatenated quantotypic (Q-) peptides representing the quantified proteins. Each CP also comprises scrambled sequences of reference (R-) peptides that relate its abundance to a single protein standard (BSA). FastCAT not only alleviates the need in purifying CP or using SDS-PAGE, but also improves the accuracy, precision and dynamic range of the absolute quantifications by grouping Q-peptides according to the expected abundance of target proteins. We benchmarked FastCAT against the reference method of MS Western and tested it in the direct molar quantifications of neurological markers in human cerebrospinal fluid at the low ng/mL level.

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Cerebrospinal Fluid

SUBMITTER: Ignacy Rzagalinski

LAB HEAD: Andrej Shevchenko

PROVIDER: PXD030728 | Pride | 2022-04-01

REPOSITORIES: Pride

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			Action	DRS
	CSF_pool_HpH_F1.raw	Raw
	CSF_pool_HpH_F1_F058188.xml	Xml
	CSF_pool_HpH_F2.raw	Raw
	CSF_pool_HpH_F2_F058190.xml	Xml
	CSF_pool_HpH_F3.raw	Raw

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FastCAT accelerates absolute quantification of proteins by using multiple short non-purified chimeric standards

Project description:Absolute (molar) quantification of proteins provides the analytical rationale for system-level modelling of diverse molecular mechanisms. FastCAT method employs multiple short (<50 kDa) stable-isotope labeled chimeric proteins (CPs) composed of concatenated quantotypic (Q-)peptides representing the quantified proteins. Each CP also comprises scrambled sequences of reference (R-)peptides that relate its abundance to a single protein standard (BSA). FastCAT not only alleviates the need in purifying CP or using SDS-PAGE, but also improves the accuracy, precision and dynamic range of the absolute quantifications by grouping Q-peptides according to the expected abundance of target proteins. We benchmarked FastCAT against the reference method of MS Western and tested it in the direct molar quantifications of neurological markers in human cerebrospinal fluid at the low ng/mL level.

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Drosophila melanogaster eye Proteomics

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The hematopoietic stem cell in chronic phase CML is characterized by a transcriptional profile resembling normal myeloid progenitor cells and reflecting loss of quiescence

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