Dataset Information

Identification and characterization of β-ODAP synthase in grass pea

ABSTRACT: Grass pea seeds and seedlings protein extracts were chromatographically fractionated. To identify the β-ODAP synthase enzyme, active fractions, as determined by a colorimetric assay that detects the presence of a derivative of free L-α,β-diaminopropionic acid (L-DAPA), were subjected to tryptic digestion and LC-MS/MS and searched against a database containing translated sequences from a long-read PacBio mRNA sequencing of grass pea seeds and seedlings.

INSTRUMENT(S): Orbitrap Fusion Lumos, Q Exactive HF, Q Exactive

ORGANISM(S): Lathyrus Sativus

TISSUE(S): Seedling, Seed

SUBMITTER: Alon Savidor

LAB HEAD: Ziv Reich

PROVIDER: PXD030847 | Pride | 2022-05-19

REPOSITORIES: Pride

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Dataset's files

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			Action	DRS
	FS1_8111_PZRE_BOS-2_1_151018_20181025020947-_1_.msf	Msf
	FS1_8111_PZRE_BOS-2_1_151018_20181025020947.raw	Raw
	FS1_8111_PZRE_BOS-3_1_151018_20181025051149-_1_.msf	Msf
	FS1_8111_PZRE_BOS-3_1_151018_20181025051149.raw	Raw
	HFX_8709_PZRE_BOS-4_1_261218-_3_.msf	Msf

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Publications

Identification and characterization of the key enzyme in the biosynthesis of the neurotoxin β-ODAP in grass pea.

Goldsmith Moshe M Barad Shiri S Knafo Maor M Savidor Alon A Ben-Dor Shifra S Brandis Alexander A Mehlman Tevie T Peleg Yoav Y Albeck Shira S Dym Orly O Ben-Zeev Efrat E Barbole Ranjit S RS Aharoni Asaph A Reich Ziv Z

The Journal of biological chemistry 20220307 5

Grass pea (Lathyrus sativus L.) is a grain legume commonly grown in Asia and Africa for food and forage. It is a highly nutritious and robust crop, capable of surviving both droughts and floods. However, it produces a neurotoxic compound, β-N-oxalyl-L-α,β-diaminopropionic acid (β-ODAP), which can cause a severe neurological disorder when consumed as a primary diet component. While the catalytic activity associated with β-ODAP formation was demonstrated more than 50 years ago, the enzyme responsi ...[more]

PMID: 35271851

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