Proteomics

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REGULATION OF THE RIBOSOME BIOGENESIS FACTOR HYVH1 BY SRC-MEDIATED PHOSPHORYLATION

ABSTRACT: In this study, we investigate cellular effects of a novel Src-mediated phosphorylation site at Tyr179 on hYVH1. We observed that this phosphorylation event attenuates hYVH1's stress granule disassembly function, enhances shuttling of hYVH1 to the nucleus, and promotes hYVH1 partitioning to the 60S ribosomal subunit. Quantitative proteomics reveal that Src co-expression with hYVH1 reduces formation of ribosomal species that represent stalled intermediates through the alteration of associating factors that promote translational repression. Collectively, these results implicate hYVH1 as a novel Src substrate and is the first demonstrated role of tyrosine phosphorylation regulating the activity of a YVH1 ortholog. Moreover, the ribosome proteome alterations point to a collaborative function of hYVH1 and Src in maintaining translational fitness.

INSTRUMENT(S): Synapt MS

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Epithelial Cell

DISEASE(S): Disease Free

SUBMITTER: Panayiotis Vacratsis  

LAB HEAD: Panayiotis Orestes Vacratsis

PROVIDER: PXD030982 | Pride | 2023-03-11

REPOSITORIES: Pride

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OV_210426_Ash_EV14a_001.msE.zip Other
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Publications

Src-mediated phosphorylation of the ribosome biogenesis factor hYVH1 affects its localization, promoting partitioning to the 60S ribosomal subunit.

DaDalt Ashley A AA   Bonham Christopher A CA   Lotze Griffin P GP   Luiso Adrian A AA   Vacratsis Panayiotis O PO  

The Journal of biological chemistry 20221110 12

Yeast VH1-related phosphatase (YVH1) (also known as DUSP12) is a member of the atypical dual-specificity phosphatase subfamily. Although no direct substrate has been firmly established, human YVH1 (hYVH1) has been shown to protect cells from cellular stressors, regulate the cell cycle, disassemble stress granules, and act as a 60S ribosome biogenesis factor. Despite knowledge of hYVH1 function, further research is needed to uncover mechanisms of its regulation. In this study, we investigate cell  ...[more]

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