Dataset Information

Inflammatory Activation Drives Re-Sculpting and Shear-Thinning of the Dendritic Cell Nucleus

ABSTRACT: To mount an adaptive immune response, dendritic cells must migrate to lymph nodes to present antigens to T cells. Critical to 3D migration is the nucleus, which is the size-limiting barrier for migration through the extracellular matrix. Here, we show that inflammatory activation of dendritic cells leads to the nucleus becoming spherically deformed and enables dendritic cells to overcome the typical 2- to 3-μm diameter limit for 3D migration through gaps in the extracellular matrix. We show that the nuclear shape change is partially attained through reduced cell adhesion, whereas improved 3D migration is achieved through reprogramming of the actin cytoskeleton. Specifically, our data point to a model whereby the phosphorylation of cofilin-1 at serine 41 drives the assembly of a cofilin-actomyosin ring proximal to the nucleus and enhances migration through 3D collagen gels. In summary, these data describe signaling events through which dendritic cells deform their nucleus and enhance their migratory capacity.

INSTRUMENT(S): Orbitrap Exploris 480

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Dendritic Cell, Blood

DISEASE(S): Bacterial Infectious Disease

SUBMITTER: Giulia Franciosa

LAB HEAD: Giulia Franciosa

PROVIDER: PXD031056 | Pride | 2024-03-14

REPOSITORIES: Pride

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Dataset's files

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			Action	DRS
	20201207_EXPL5_EvoPRI_GF_30SPD_DIA_Prot_EPIC-XS_van_den_Bogaart_human_moDC-Donor0_Ctrl_R1.raw	Raw
	20201207_EXPL5_EvoPRI_GF_30SPD_DIA_Prot_EPIC-XS_van_den_Bogaart_human_moDC-Donor0_Ctrl_R2.raw	Raw
	20201207_EXPL5_EvoPRI_GF_30SPD_DIA_Prot_EPIC-XS_van_den_Bogaart_human_moDC-Donor0_LPS-4h_R2.raw	Raw
	20201207_EXPL5_EvoPRI_GF_30SPD_DIA_Prot_EPIC-XS_van_den_Bogaart_human_moDC_Donor0_LPS-4h_R1.raw	Raw
	20201219_EXPL5_EvoPRI_GF_60SPD_DIA_Phos_EPIC-XS_van_den_Bogaart_human_moDC-Donor0_Ctrl_R1.raw	Raw

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Publications

Atypical cofilin signaling drives dendritic cell migration through the extracellular matrix via nuclear deformation.

Warner Harry H Franciosa Giulia G van der Borg Guus G Coenen Britt B Faas Felix F Koenig Claire C de Boer Rinse R Classens René R Maassen Sjors S Baranov Maksim V MV Mahajan Shweta S Dabral Deepti D Bianchi Frans F van Hilten Niek N Risselada Herre Jelger HJ Roos Wouter H WH Olsen Jesper Velgaard JV Cano Laia Querol LQ van den Bogaart Geert G

Cell reports 20240227 3

To mount an adaptive immune response, dendritic cells must migrate to lymph nodes to present antigens to T cells. Critical to 3D migration is the nucleus, which is the size-limiting barrier for migration through the extracellular matrix. Here, we show that inflammatory activation of dendritic cells leads to the nucleus becoming spherically deformed and enables dendritic cells to overcome the typical 2- to 3-μm diameter limit for 3D migration through gaps in the extracellular matrix. We show that ...[more]

PMID: 38416638

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