Dataset Information

A universal and cost-effective method for general and specific RNA-protein complex isolation_RBPome

ABSTRACT: The establishment of a protocol isolating specific RNPs by combining a total RNP isolation with an antisense probe-mediated specific RNP isolation

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Arabidopsis Thaliana (mouse-ear Cress)

TISSUE(S): Leaf, Diploid Cell

SUBMITTER: Roosje Van Ende

LAB HEAD: Koen Geuten

PROVIDER: PXD031578 | Pride | 2023-05-10

REPOSITORIES: Pride

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Dataset's files

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			Action	DRS
	B08468_Bp_WNE12_trap2_PRC-5259_Geuten_cleanup_shotgun-1_rerun.raw	Raw
	B08470_Bp_WNE12_trap2_PRC-5259_Geuten_cleanup_shotgun-2.raw	Raw
	B08472_Bp_WNE12_trap2_PRC-5259_Geuten_cleanup_shotgun-3.raw	Raw
	B08474_Bp_WNE12_trap2_PRC-5259_Geuten_cleanup_shotgun-4.raw	Raw
	B08476_Bp_WNE12_trap2_PRC-5259_Geuten_cleanup_shotgun-5.raw	Raw

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Publications

A widely applicable and cost-effective method for specific RNA-protein complex isolation.

Balzarini Sam S Van Ende Roosje R Voet Arnout A Geuten Koen K

Scientific reports 20230427 1

Although methodological advances have been made over the past years, a widely applicable, easily scalable and cost-effective procedure that can be routinely used to isolate specific ribonucleoprotein complexes (RNPs) remains elusive. We describe the "Silica-based Acidic Phase Separation (SAPS)-capture" workflow. This versatile method combines previously described techniques in a cost-effective, optimal and widely applicable protocol. The specific RNP isolation procedure is performed on a pre-pur ...[more]

PMID: 37106019

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