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Proximity labeling of SPARK-TurboID

ABSTRACT: SPARK is an essential AGC kinase found in apicomplexan parasites, ubiquitous pathogens of humans. Previously, we showed that SPARK is necessary for parasite calcium mobilization, invasion, and egress from host cells. Here, we perform proximity labeling experiments to determine proteins sharing subcellular proximity with SPARK in live intracellular parasites.

INSTRUMENT(S): Orbitrap Exploris 480

ORGANISM(S): Toxoplasma Gondii Gt1

SUBMITTER: Alice Herneisen

LAB HEAD: Sebastian Lourido

PROVIDER: PXD039983 | Pride | 2024-07-12

REPOSITORIES: Pride

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			Action	DRS
	20220424_tgALH184_intracellular_DDA_20220424154404.raw	Raw
	20220424_tgALH293_intracellular_DDA.raw	Raw
	20220729_tgALH184_intracellular_DDA.raw	Raw
	20220729_tgALH293_intracellular_DDA.raw	Raw
	20220731_tgALH184_intracellular_DDA.raw	Raw

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Proximity labeling of TurboID-SPARKEL

Project description:Toxoplasma gondii is a ubiquitous parasite of animals. We have previously shown that T. gondii possesses an AGC kinase, SPARK, that is necessary for calcium mobilization, invasion, and egress from host cells. Interaction studies have revealed that SPARK complexes with an elongin-like protein, which we term SPARKEL. Here, we performed proximity labeling of parasites expressing TurboID-SPARKEL endogenously.

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