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Proximity labeling of TurboID-SPARKEL

ABSTRACT: Toxoplasma gondii is a ubiquitous parasite of animals. We have previously shown that T. gondii possesses an AGC kinase, SPARK, that is necessary for calcium mobilization, invasion, and egress from host cells. Interaction studies have revealed that SPARK complexes with an elongin-like protein, which we term SPARKEL. Here, we performed proximity labeling of parasites expressing TurboID-SPARKEL endogenously.

INSTRUMENT(S): Orbitrap Exploris 480

ORGANISM(S): Toxoplasma Gondii Gt1

SUBMITTER: Alice Herneisen

LAB HEAD: Sebastian Lourido

PROVIDER: PXD039986 | Pride | 2024-07-12

REPOSITORIES: Pride

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			Action	DRS
	20221222_tgALH184_DDA_R1.raw	Raw
	20221222_tgALH184_DDA_R2.raw	Raw
	20221222_tgALH493_DDA_R1.raw	Raw
	20221222_tgALH493_DDA_R2.raw	Raw
	tgALH493_intracellular_DDA.mzML	Mzml

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Proximity labeling of SPARKEL-TurboID

Project description:Toxoplasma gondii is a ubiquitous parasite of animals. We have previously shown that T. gondii possesses an AGC kinase, SPARK, that is necessary for calcium mobilization, invasion, and egress from host cells. Interaction studies have revealed that SPARK complexes with an elongin-like protein, which we term SPARKEL. Here, we performed proximity labeling of parasites expressing SPARKEL-TurboID endogenously.

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