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Paleoproteomics of ancient human teeth

ABSTRACT: Teeth are a well-known source of information for paleoanthropologists. Here, we established the ancient dental metaproteomes in several samples from historic sites. The shotgun metaproteomics analysis relies on a iterative search strategy for the identification of the proteins and their origins.

INSTRUMENT(S): Orbitrap Exploris 480

ORGANISM(S): Homo Sapiens (human) Archaea Viruses Bacteria Eukaryota (eucaryotes)

TISSUE(S): Tooth

SUBMITTER: Jean ARMENGAUD

LAB HEAD: Jean Armengaud

PROVIDER: PXD031786 | Pride | 2023-03-30

REPOSITORIES: Pride

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Dataset's files

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			Action	DRS
	E00113_MS21-012_Te-a.raw	Raw
	E00114_MS21-012_Te-b.raw	Raw
	E00115_MS21-012_Te-c.raw	Raw
	E00115_MS21-012_Te-c_E00117_MS21-012_Te-e_E00116_MS21-012_Te-d_E00114_MS21-012_Te-b_E00113_MS21-012_Te-a.mgf	Mgf
	E00116_MS21-012_Te-d.raw	Raw

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Publications

An ancient coronavirus from individuals in France, circa 16th century.

Oumarou Hama Hamadou H Chenal Thomas T Pible Olivier O Miotello Guylaine G Armengaud Jean J Drancourt Michel M

International journal of infectious diseases : IJID : official publication of the International Society for Infectious Diseases 20230315

<h4>Objectives</h4>At the time when the COVID-19 pandemic was responsible for more than six million deaths worldwide, the antiquity of coronaviruses remains undefined. We investigated individuals buried during the 16th century in France for the direct and paleoserological diagnosis of the coronavirus.<h4>Methods</h4>The 2011-2012 excavation of Abbey Saint-Pierre in Baume-Les-Messieurs, France uncovered 12 skeletons of individuals from the 13th to the 18th century. The total proteins extracted fr ...[more]

PMID: 36924840

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Extended paleoproteomics of ancient human teeth

Project description:Teeth are a well-known source of information for paleoanthropologists. Here, we established the ancient dental metaproteomes in several samples from historic sites. The shotgun metaproteomics analysis relies on a iterative search strategy for the identification of the proteins and their origins.

2023-03-30 | PXD031802 | Pride

Ancient DNA teeth

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Mass spectrometry imaging of ancient teeth proteomes

Project description:In order to research the variation in protein distribution in teeth, proteins were extracted from archaeological (15-18th century, Netherlands) and modern teeth and identified using LC-MS/MS. Of the recovered proteins we then visualised the distribution of collagen type I (both the alpha-1 and -2 chains), alpha-2-HS-glycoprotein, haemoglobin subunit alpha and myosin light polypeptide 6 using MALDI-MSI. We found distinct differences in the spatial distributions of different proteins as well as between some peptides of the same protein. The reason for these differences in protein spatial distribution remain unclear, yet this study highlights the ability of MALDI-MSI for visualisng the spatial distribution of proteins in archaeological biomineralised tissues. Therefore MALDI-MSI might prove a useful tool to improve our understanding of protein preservation as well as aid in deciding sampling strategies.

2023-03-11 | PXD038114 | Pride

Flash proteotyping of microbial isolates by tandem mass spectrometry

Project description:Flash proteotyping is a methodology for ultra-fast identification of microorganisns by tandem mass spectrometry. Here, we obtained results on five reference strains and ten new bacterial isolates. The methodology is based on direct sample infusion into the mass spectromete and an original, highly sensitive procedure for data processing and taxonomic identification.

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Comparative transcriptome profiles of human dental pulp cells from maxillary and mandibular teeth

Project description:PITX1 had a significantly higher expression in the lower teeth compared to the upper teeth and this difference in PITX1 level was more evident in the molars compared to premolars, consistent with data in mouse developing teeth. These show that the differential gene expression during odontogenesis can continue to exist in mature teeth. We suggest that an in vitro study of dental pulp cells should take the differential gene profiles between the mandibular and maxillary teeth into consideration. The knowledge about gene profiling and pathways in mature teeth paves a way to explore a more precise treatment approach in regenerative dentistry.

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Protein Extraction from Ancient Teeth and Dental Calculus Compatible with Subsequent DNA Extraction

Project description:Dental calculus is becoming a crucial material in the study of past populations, with increasing interest in its proteomic and genomic content. Here we suggest further development of protocol for analysis of ancient proteins and a combined approach for subsequent ancient DNA extraction. We tested the protocol on recent teeth. We then applied the optimised protocol to ancient teeth to limit the destruction of calculus, as it is a precious and irreplaceable source of dietary, microbiological, and ecological information in the archaeological context. Finally, the applicability of the protocol was proven on samples of ancient calculus.

2023-09-11 | PXD042668 | Pride

Comparative gene expression analysis of the human dental pulp in deciduous and permanent teeth

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2014-08-21 | E-GEOD-51449 | biostudies-arrayexpress

limpet teeth LC-MSMS - The Microstructure, Proteomics and Crystallization of the Limpet Teeth

Project description:Limpets are marine mollusks using mineralized teeth, one of the hardest and strongest biomaterials, to feed on algae on intertidal rocks. However, most of studies only focuses on the ultrastructure and chemical composition of the teeth while the molecular information is largely unknown, limiting our understanding of this unique and fundamental biomineralization process. In this study, we investigated the teeth of limpet Cellana toreuma from three perspectives: 1) by using electron microscopy to observe the microstructure of the teeth; 2) by using proteomics and RNA-seq to investigate the proteins involved in the limpet teeth and 3) by in vitro crystallization experiment combined with Raman spectroscopy to investigate the effects of proteins and chitin framework on crystal formation. It is found that the limpets formed alternatively tricuspid teeth and unicuspid teeth. Small nanoneedles were densely packed at the tips or leading regions of the cusps. In contrast, big nanoneedles resembling chemical synthesized goethite were loosely packed in the trailing regions of the cusps. Proteins extracted from the whole teeth such as ferritin, peroxiredoxin, arginine kinase, GTPase-Rabs and clathrin were identified by proteomics. Goethite-binding experiment coupled with proteomics and RNA-seq highlighted six chitin-binding proteins (CtCBPs). Furthermore, these proteins or the framework chitin only induced packing of crystals without affecting their crystal polymorphs in vitro. Taken together, the limpets formed hierarchical teeth across different length scales through preformed framework and secreted complex proteins; in addition, the chitin could also be an important player in controlling crystallinity and crystal packing in vivo. This study provides insight into the unique biomineralization process in the limpet teeth at the molecular levels, which may guide biomimetic strategies aimed at designing hard materials at room temperature.

2019-03-26 | PXD010113 | Pride

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