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Identification of gamma-carboxyglutamic acid residues in mouse ERGP by LC-MS/MS.

ABSTRACT: Vitamin K (VK) is a micronutrient required for the gamma-carboxylation of secreted proteins. Clinical studies have recently implicated VK in the pathophysiology of diabetes. Our data indicate that rodent beta-cells lacking gamma-carboxylation fail to adapt their insulin secretion in response to glucose in the context of age-related insulin resistance or diet-induced beta-cell stress. In contrast, VK supplementation protects beta-cells from endoplasmic reticulum stress-induced apoptosis. Using anti-Gla immunoprecipitation, we identified ERGP as a putative gamma-carboxylated protein present in pancreatic beta-cells. We used LC-MS/MS to confirm the presence of gamma-carboxyglutamic residues in ERGP.

INSTRUMENT(S): Orbitrap Fusion

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Cell Culture

SUBMITTER: Mathieu Ferron

LAB HEAD: Mathieu Ferron

PROVIDER: PXD032955 | Pride | 2023-05-10

REPOSITORIES: Pride

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			Action	DRS
	2019_7_41_48_AM_File_Size__735263192__Byte___F037093_.mzid.gz	Mzid
	2019_7_41_48_AM_File_Size__735263192__Byte___F037093_.mzid_2019_7_41_48_AM_File_Size__735263192__Byte___F037093_.MGF	Mzid
	OF_20190814_FER_03.raw	Raw
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