Proteomics

Dataset Information

0

HDX-MS Analysis of the Rab3GAP1/Rab3GAP2

ABSTRACT: An HDX-MS analysis of the Rab3GAP1 and Rab3GAP2 subunit interface, and the binding interface between Rab18 and the full Rab3GAP1/2 complex.

INSTRUMENT(S): impact HD

ORGANISM(S): Homo Sapiens (human)

SUBMITTER: John Burke  

LAB HEAD: Dr. John E. Burke

PROVIDER: PXD033072 | Pride | 2025-03-10

REPOSITORIES: Pride

Json Xml

Dataset's files

Source:
Action DRS
01_25_22_MPHDX13_RG12R_MSMS_0_01_18621.d.mzXML Mzxml
01_25_22_MPHDX13_RG12R_MSMS_0_01_18621.d.zip Other
01_25_22_MPHDX13_RG12_MSMS_0_01_18619.d.mzXML Mzxml
01_25_22_MPHDX13_RG1_MSMS_0_01_18617.d.mzXML Mzxml
01_25_22_MPHDX13_RG1_MSMS_0_01_18617.d.zip Other
Items per page:
1 - 5 of 68
altmetric image

Publications

Biochemical and structural characterization of Rab3GAP reveals insights into Rab18 nucleotide exchange activity.

Fairlie Gage M J GMJ   Nguyen Kha M KM   Nam Sung-Eun SE   Shaw Alexandria L AL   Parson Matthew A H MAH   Shariati Hannah R HR   Wang Xinyin X   Jenkins Meredith L ML   Gong Michael M   Burke John E JE   Yip Calvin K CK  

Nature communications 20250108 1

The heterodimeric Rab3GAP complex is a guanine nucleotide exchange factor (GEF) for the Rab18 GTPase that regulates lipid droplet metabolism, ER-to-Golgi trafficking, secretion, and autophagy. Why both subunits of Rab3GAP are required for Rab18 GEF activity and the molecular basis of how Rab3GAP engages and activates its cognate substrate are unknown. Here we show that human Rab3GAP is conformationally flexible and potentially autoinhibited by the C-terminal domain of its Rab3GAP2 subunit. Our h  ...[more]

Similar Datasets

Proteomics Detailing the Structural and Dynamic Interactions Between WDR44 and Rab11
2023-03-11 | PXD035741 | Pride
Proteomics Structure of the phosphoinositide 3-kinase p110-p101 complex reveals molecular mechanism of GPCR activation
2021-09-22 | PXD025209 | Pride
Proteomics Characterization of m-lipin using HDX-MS
2021-06-29 | PXD022172 | Pride
Proteomics ATP-competitive and allosteric inhibitors induce differential conformational changes at the autoinhibitory interface of Akt
2024-08-23 | PXD039028 | Pride
Proteomics HDX-MS analysis of PI4Ka trimer and FAM126a (415-521) with calcineurin
2024-09-03 | PXD050633 | Pride
Proteomics HDX-MS Analysis of CeSpartin and CtSpartinL
2023-12-27 | PXD043175 | Pride
Proteomics HDX-MS Analysis of the protein ALT51
2023-02-22 | PXD034850 | Pride
Proteomics HDX-MS Analysis of the PI4K EFR3A Complex
2025-03-10 | PXD043442 | Pride
Proteomics Using HDX-MS to study Rab and Ral covalently bound to MRTX1257
2025-03-10 | PXD041440 | Pride
Proteomics Defining the molecular mechanism of PI4KA complex regulation by the phosphatase Calcineurin
2024-09-03 | PXD043409 | Pride