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Title（Project name）Proteomic Investigation of the Molecular Mechanisms in Response to the PEG-Induced Drought Stress in Soybean Roots

ABSTRACT: Roots are generally the critical drought sensors, but little is known about their molecular response to drought stress. We used the drought-tolerant soybean variety ‘Jiyu 47’ to investigate the differentially expressed proteins (DEPs) in soybean roots during the seedling stage based on the TMT proteomics analysis. Results of enrichment analyses based on a total of 468 DEPs revealed a coordinated expression pattern of proteins involved in various cellular metabolisms responding to drought stress in soybean roots. Our results showed that drought stress caused significant alterations in the expression of proteins involved in several metabolic pathways in soybean roots, including the carbohydrate metabolism, the metabolism of the osmotic regulation substances, and the antioxidant defense system (i.e., the glutathione metabolism). Increased production of reduced glutathione (GSH) enhanced the prevention of the damage caused by reactive oxygen species and the tolerance of the abiotic stress. The glutathione metabolism played a key role in modifying the antioxidant defense system in response to drought stress in soybean roots. Our proteomic study demonstrated that the soybean plants responded to drought stress by coordinating their protein expression during the vegetative stage, providing novel insights into the molecular mechanisms regulating the response to abiotic stress in plants.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Glycine Max

TISSUE(S): Root

SUBMITTER: Ying Zhou

LAB HEAD: Ying Zhou

PROVIDER: PXD033092 | Pride | 2022-05-08

REPOSITORIES: Pride

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			Action	DRS
	KA247TQ_01.raw	Raw
	KA247TQ_02.raw	Raw
	KA247TQ_03.raw	Raw
	KA247TQ_04.raw	Raw
	KA247TQ_05.raw	Raw

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