Dataset Information

Label-free quantitative proteomics analysis of Drosophila melanogaster lethal giant larvae mutants reveals potential therapeutic effects of melatonin

ABSTRACT: The present study is a pioneering investigation that has been carefully designed to study proteomic effects of specific cancer-related mutation using the lgl mutant of D. melanogaster that has not been well characterised thus far. Recent findings concerning the D. melanogaster cancer models suggest that this model is well suited for study of cancer mechanisms and therapeutic interventions, especially in the brain and intestines (Mirzoyan et al., 2019). In addition, the integration of circadian biology into cancer research offers new insights into the implications of optimal timing for cancer treatment that could potentially reduce undesirable side effects and enhance prognosis (Ballesta et al., 2017). As a result, label free quantitative mass spectrometry was employed to investigate tissue-specific protein expression in head and intestinal tissues of D. melanogaster lgl mutants which was collected at 6-hours intervals throughout the 24-hour period. Furthermore, the efficacy of melatonin as an anticancer agent at the proteome level was also investigated. Together, the resulting information could potentially identify malfunctioning signalling pathways and circadian rhythm-dependent protein markers during tumorigenesis, as well as the mechanism of actions of melatonin on these processes, post-treatment

INSTRUMENT(S): Orbitrap Fusion

ORGANISM(S): Drosophila Melanogaster (fruit Fly)

SUBMITTER: Jaime Jayapalan

LAB HEAD: Jaime Jacqueline Jayapalan

PROVIDER: PXD033191 | Pride | 2023-05-10

REPOSITORIES: Pride

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Label-free quantitative mass spectrometry analysis of the circadian proteome of Drosophila melanogaster lethal giant larvae mutants reveals potential therapeutic effects of melatonin.

Wong Kar-Cheng KC Jayapalan Jaime J JJ Subramanian Perumal P Ismail Mohd Nazri MN Abdul-Rahman Puteri S PS

Archives of insect biochemistry and physiology 20230313 2

Mutation in the Drosophila melanogaster lethal giant larvae (lgl), a tumor suppressor gene with a well-established role in cellular polarity, is known to results in massive cellular proliferation and neoplastic outgrowths. Although the tumorigenic properties of lgl mutant have been previously studied, however, little is known about its consequences on the proteome. In this study, mass spectrometry-based label-free quantitative proteomics was employed to investigate the changes in the head and in ...[more]

PMID: 36915983

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	H00LGLM_1.raw	Raw
	H00LGLM_2.raw	Raw
	H00LGLM_3.raw	Raw
	H00LGL_1.raw	Raw
	H00LGL_2.raw	Raw