Combinatorial screening of biological degrader modules to destroy intractable proteins – nonkinetic data
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ABSTRACT: Abstract – nonkinetic data Targeted protein degradation is a potent strategy against intracellular proteins impervious to traditional drugs. We describe a platform reliant on high-throughput cloning and mRNA-based delivery to quickly screen 100s-1000s modular biological degraders which we challenge against the intractable and high-turnover c-Myc oncoprotein. We uncover critical principles to drive the discovery of degraders against any target, capable of operating without prior cell line engineering, with direct applications in research and potentially therapy.
INSTRUMENT(S): timsTOF Pro 2
ORGANISM(S): Homo Sapiens (human)
TISSUE(S): Cell Culture, Colon Epithelial Cell
DISEASE(S): Colon Cancer
SUBMITTER: Sonja Hess
LAB HEAD: Sonja Hess
PROVIDER: PXD033221 | Pride | 2023-11-14
REPOSITORIES: Pride
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