Proteomics

Dataset Information

0

Combinatorial screening of biological degrader modules to destroy intractable proteins – nonkinetic data


ABSTRACT: Abstract – nonkinetic data Targeted protein degradation is a potent strategy against intracellular proteins impervious to traditional drugs. We describe a platform reliant on high-throughput cloning and mRNA-based delivery to quickly screen 100s-1000s modular biological degraders which we challenge against the intractable and high-turnover c-Myc oncoprotein. We uncover critical principles to drive the discovery of degraders against any target, capable of operating without prior cell line engineering, with direct applications in research and potentially therapy.

INSTRUMENT(S): timsTOF Pro 2

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Cell Culture, Colon Epithelial Cell

DISEASE(S): Colon Cancer

SUBMITTER: Sonja Hess  

LAB HEAD: Sonja Hess

PROVIDER: PXD033221 | Pride | 2023-11-14

REPOSITORIES: Pride

Dataset's files

Source:
Action DRS
20210414_220339_DOA078_Protac_IVEB_Trim21_041421.sne Other
DIA.zip Other
DOA078_Protac_IVEB_Trim21_DDA_DIAHybri_041121.kit Other
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