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Combinatorial screening of biological degrader modules to destroy intractable proteins – Kinetic Data

ABSTRACT: Abstract – kinetic data Targeted protein degradation is a potent strategy against intracellular proteins impervious to traditional drugs. We describe a platform reliant on high-throughput cloning and mRNA-based delivery to quickly screen 100s-1000s modular biological degraders which we challenge against the intractable and high-turnover c-Myc oncoprotein. We uncover critical principles to drive the discovery of degraders against any target, capable of operating without prior cell line engineering, with direct applications in research and potentially therapy.

INSTRUMENT(S): timsTOF Pro 2

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Cell Culture, Colon Epithelial Cell

DISEASE(S): Colon Cancer

SUBMITTER: Sonja Hess

LAB HEAD: Sonja Hess

PROVIDER: PXD033222 | Pride | 2023-11-14

REPOSITORIES: Pride

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			Action	DRS
	20210415_061445_DOA078_041021_Protac_IVEB_Trim21_kinetic_HybridLib0411_041421.sne	Other
	DIA_kinetic.zip	Other
	DOA078_041021_Protac_IVEB_Trim21_kinetic_DDA.kit	Other

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