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PhosphoProtein-Peptide Turnover Profiling, exploratory

ABSTRACT: We combine pulsed stable isotope labeling of amino acids (pSILAC) with enrichment of phosphorylated peptides in a method we term PhosphoProtein-Peptide Turnover Profiling (PhosphoPPToP). We apply the method to HeLa cells to find peptides whose clearance rates differ from the protein median. Using theoretical modelling, we show that readouts from PPToP are primarily defined by the wiring of the PTM-modification network and not by effects of the PTM on a protein's proteolytic stability. Thus PPToP can be used to identify PTMs occurring, e.g., preferentially early or late in a protein's lifetime. This enables identification of, e.g., protein maturation or protein complex assembly intermediates.

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Permanent Cell Line Cell, Cell Culture

DISEASE(S): Cervix Carcinoma

SUBMITTER: Henrik Hammarén

LAB HEAD: Mikhail Savitski

PROVIDER: PXD033254 | Pride | 2022-12-02

REPOSITORIES: Pride

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Dataset's files

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			Action	DRS
	MQ_Phospho_search.zip	Other
	MQ_Phospho_txt.zip	Other
	MQ_Total_search.zip	Other
	MQ_Total_txt.zip	Other
	Sample_mapping.csv	Csv

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