Proteomics

Dataset Information

0

Digital mapping of immunopeptidomes: SWATH-MS


ABSTRACT: We present a novel mass spectrometry-based high-throughput workflow and an open-source computational and data resource to reproducibly identify and quantify HLA-associated peptides. Collectively, the resources support the generation of HLA allele-specific peptide assay libraries consisting of consensus fragment ion spectra, and the analysis of quantitative digital maps of HLA peptidomes generated by SWATH mass spectrometry (MS) from a range a biological sources. This study represents the first community-based effort to develop a robust platform for the reproducible and quantitative measurement of the entire repertoire of peptides presented by HLA molecules, an essential step towards the design of efficient immunotherapies.

INSTRUMENT(S): TripleTOF 5600

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Cell Suspension Culture, Peripheral Blood Mononuclear Cell

SUBMITTER: Etienne Caron  

LAB HEAD: Ruedi Aebersold

PROVIDER: PXD001904 | Pride | 2015-07-14

REPOSITORIES: Pride

Dataset's files

Source:
altmetric image

Publications


We present a novel mass spectrometry-based high-throughput workflow and an open-source computational and data resource to reproducibly identify and quantify HLA-associated peptides. Collectively, the resources support the generation of HLA allele-specific peptide assay libraries consisting of consensus fragment ion spectra, and the analysis of quantitative digital maps of HLA peptidomes generated from a range of biological sources by SWATH mass spectrometry (MS). This study represents the first  ...[more]

Similar Datasets

2017-03-09 | PXD003972 | Pride
2015-07-14 | PXD001872 | Pride
2022-05-31 | PXD033935 | Pride
2018-05-10 | PXD007596 | Pride
2019-10-14 | PXD011628 | Pride
2021-04-16 | PXD019643 | Pride
2019-10-14 | PXD015748 | Pride
2021-04-16 | PXD020186 | Pride
2018-03-01 | PXD008937 | Pride
2020-12-01 | PXD019258 | Pride